<
WB (Western Blot)
(Western blot analysis of GRP78 expression in HepG2 (A); HeLa (B); Raw264.7 (C); mouse brain (D) whole cell lysates.)
Rabbit HSPA5/GRP78/BiP Polyclonal Antibody | anti-HSPA5/GRP78/BiP antibody
Anti-HSPA5/GRP78/BiP Antibody (C-Terminus)
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
HumanPredicted: Mouse, Monkey
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunoaffinity Purified
Synonyms
HSPA5/GRP78/BiP, Antibody; Anti-HSPA5/GRP78/BiP Antibody (C-Terminus); Rabbit Polyclonal to Human HSPA5/GRP78/BiP; HSPA5; BIP; Heat shock 70 kDa protein 5; MIF2; GRP-78; GRP78; anti-HSPA5/GRP78/BiP antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse, Monkey
Predicted: Mouse, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GRP78 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Sequence Length
654
Applicable Applications for anti-HSPA5/GRP78/BiP antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human GRP78.
Conjugation
Unconjugated
Epitope
C-Terminus
Preparation and Storage
Store at -20 degree C.
Aliquot to avoid freeze/thaw cycles.
Aliquot to avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of GRP78 expression in HepG2 (A); HeLa (B); Raw264.7 (C); mouse brain (D) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of GRP78 expression in HepG2 (A); HeLa (B); Raw264.7 (C); mouse brain (D) whole cell lysates.)
IF (Immunofluorescence)
(Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohiostchemistry)
(Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-HSPA5/GRP78/BiP antibody
Probably plays a role in facilitating the assembly of multimericprotein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins viaits interaction with DNAJC10, probably to facilitate the release ofDNAJC10 from its substrate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,333 Da
NCBI Official Full Name
endoplasmic reticulum chaperone BiP
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 5
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78; HEL-S-89n
NCBI Protein Information
endoplasmic reticulum chaperone BiP
UniProt Protein Name
Endoplasmic reticulum chaperone BiP
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
HSP70 family protein 5Curated
Similar Products
Product Notes
The HSPA5/GRP78/BiP hspa5 (Catalog #AAA162325) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HSPA5/GRP78/BiP Antibody (C-Terminus) reacts with Human Predicted: Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA5/GRP78/BiP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the HSPA5/GRP78/BiP hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA5/GRP78/BiP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.