IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HNF4A Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit HNF4A Polyclonal Antibody | anti-HNF4A antibody
HNF4A Antibody (N-term)
Gene Names
HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Xenopus)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HNF4A, Antibody; HNF4A Antibody (N-term); Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4; HNF4A; HNF4; NR2A1; TCF14; anti-HNF4A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-139 amino acids from the N-terminal region of human HNF4A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
107-139
Sequence Length
395
Applicable Applications for anti-HNF4A antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HNF4A Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of HNF4A Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of HNF4A Antibody (N-term) in 293 cell line lysates (35ug/lane). HNF4A (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of HNF4A Antibody (N-term) in 293 cell line lysates (35ug/lane). HNF4A (arrow) was detected using the purified Pab.)
Related Product Information for anti-HNF4A antibody
HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines.
Product Categories/Family for anti-HNF4A antibody
References
Koh, W., et al. BMC Genomics 11 SUPPL 1, S6 (2010)
Wang, F., et al. Chin. Med. J. 122(20):2477-2482(2009)
Taghavi, S.M., et al. Cardiovasc Diabetol 8, 63 (2009)
Guo, H., et al. Biochem. J. 394 (PT 2), 379-387 (2006)
Hong, Y.H., et al. J. Biol. Chem. 278(30):27495-27501(2003)
Wang, F., et al. Chin. Med. J. 122(20):2477-2482(2009)
Taghavi, S.M., et al. Cardiovasc Diabetol 8, 63 (2009)
Guo, H., et al. Biochem. J. 394 (PT 2), 379-387 (2006)
Hong, Y.H., et al. J. Biol. Chem. 278(30):27495-27501(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52785
NCBI Official Full Name
hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
NCBI Official Synonym Full Names
hepatocyte nuclear factor 4, alpha
NCBI Official Symbol
HNF4A
NCBI Official Synonym Symbols
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
NCBI Protein Information
hepatocyte nuclear factor 4-alpha
UniProt Protein Name
Hepatocyte nuclear factor 4-alpha
UniProt Gene Name
HNF4A
UniProt Synonym Gene Names
HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
UniProt Entry Name
HNF4A_HUMAN
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Product Notes
The HNF4A hnf4a (Catalog #AAA287283) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 107-139. The HNF4A Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's HNF4A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HNF4A hnf4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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