FCM/FACS (Flow Cytometry)
(HMGA2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit HMGA2 Polyclonal Antibody | anti-HMGA2 antibody
HMGA2 Antibody (C-term)
Gene Names
HMGA2; BABL; LIPO; HMGIC; HMGI-C; STQTL9
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HMGA2, Antibody; HMGA2 Antibody (C-term); High mobility group protein HMGI-C; High mobility group AT-hook protein 2; HMGA2; HMGIC; anti-HMGA2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HMGA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the C-terminal region of human HMGA2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
64-92
Sequence Length
90
Applicable Applications for anti-HMGA2 antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HMGA2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HMGA2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(HMGA2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGA2 antibody detected the HMGA2 protein (arrow).)
WB (Western Blot)
(HMGA2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGA2 antibody detected the HMGA2 protein (arrow).)
Related Product Information for anti-HMGA2 antibody
This gene encodes a protein that belongs to the
non-histone chromosomal high mobility group (HMG) protein family.
HMG proteins function as architectural factors and are essential
components of the enhancesome. This protein contains structural
DNA-binding domains and may act as a transcriptional regulating
factor. Identification of the deletion, amplification, and
rearrangement of this gene that are associated with myxoid
liposarcoma suggests a role in adipogenesis and mesenchymal
differentiation. A gene knock out study of the mouse counterpart
demonstrated that this gene is involved in diet-induced obesity.
Alternate transcriptional splice variants, encoding different
isoforms, have been characterized.
non-histone chromosomal high mobility group (HMG) protein family.
HMG proteins function as architectural factors and are essential
components of the enhancesome. This protein contains structural
DNA-binding domains and may act as a transcriptional regulating
factor. Identification of the deletion, amplification, and
rearrangement of this gene that are associated with myxoid
liposarcoma suggests a role in adipogenesis and mesenchymal
differentiation. A gene knock out study of the mouse counterpart
demonstrated that this gene is involved in diet-induced obesity.
Alternate transcriptional splice variants, encoding different
isoforms, have been characterized.
Product Categories/Family for anti-HMGA2 antibody
References
Mu, G., et al. Hum. Pathol. 41(4):493-502(2010)
Pillas, D., et al. PLoS Genet. 6 (2), E1000856 (2010) :
Yang, T.L., et al. Ann. Hum. Genet. 74(1):11-16(2010)
Wei, J.J., et al. Am. J. Surg. Pathol. 34(1):18-26(2010)
Tay, Y., et al. Stem Cell Rev 5(4):328-333(2009)
Schwanbeck, R., et al. J. Biol. Chem. 275(3):1793-1801(2000)
Chau, K.Y., et al. Nucleic Acids Res. 23(21):4262-4266(1995)
Schoenmakers, E.F., et al. Nat. Genet. 10(4):436-444(1995)
Ashar, H.R., et al. Cell 82(1):57-65(1995)
Schoenmakers, E.F., et al. Genes Chromosomes Cancer 11(2):106-118(1994)
Manfioletti, G., et al. Nucleic Acids Res. 19(24):6793-6797(1991)
Pillas, D., et al. PLoS Genet. 6 (2), E1000856 (2010) :
Yang, T.L., et al. Ann. Hum. Genet. 74(1):11-16(2010)
Wei, J.J., et al. Am. J. Surg. Pathol. 34(1):18-26(2010)
Tay, Y., et al. Stem Cell Rev 5(4):328-333(2009)
Schwanbeck, R., et al. J. Biol. Chem. 275(3):1793-1801(2000)
Chau, K.Y., et al. Nucleic Acids Res. 23(21):4262-4266(1995)
Schoenmakers, E.F., et al. Nat. Genet. 10(4):436-444(1995)
Ashar, H.R., et al. Cell 82(1):57-65(1995)
Schoenmakers, E.F., et al. Genes Chromosomes Cancer 11(2):106-118(1994)
Manfioletti, G., et al. Nucleic Acids Res. 19(24):6793-6797(1991)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11832
NCBI Official Full Name
high mobility group protein HMGI-C isoform a
NCBI Official Synonym Full Names
high mobility group AT-hook 2
NCBI Official Symbol
HMGA2
NCBI Official Synonym Symbols
BABL; LIPO; HMGIC; HMGI-C; STQTL9
NCBI Protein Information
high mobility group protein HMGI-C
UniProt Protein Name
High mobility group protein HMGI-C
UniProt Gene Name
HMGA2
UniProt Synonym Gene Names
HMGIC
UniProt Entry Name
HMGA2_HUMAN
Similar Products
Product Notes
The HMGA2 hmga2 (Catalog #AAA289806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 64-92. The HMGA2 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's HMGA2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the HMGA2 hmga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.