WB (Western Blot)
(Histone macroH2A1 pAb tested by Western blot. HeLa nuclear extract (20 ug per lane) was probed with Histone macroH2A1 pAb at a dilution of 1:2,000.)
Rabbit anti-Human, Mouse Histone macroH2A1 Polyclonal Antibody | anti-macroH2A1 antibody
Histone macroH2A1 antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Synonyms
Histone macroH2A1, Antibody; Histone macroH2A1 antibody (pAb); anti-macroH2A1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-macroH2A1 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
This Histone macroH2A1 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse macroH2A1.2, so will react with macroH2A1 and the macroH2A1.1 and macroH2A1.2 isoforms.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Histone macroH2A1 pAb tested by Western blot. HeLa nuclear extract (20 ug per lane) was probed with Histone macroH2A1 pAb at a dilution of 1:2,000.)
WB (Western Blot)
(Histone macroH2A1 pAb tested by Western blot. HeLa nuclear extract (20 ug per lane) was probed with Histone macroH2A1 pAb at a dilution of 1:2,000.)
IF (Immunofluorescence)
(Histone macroH2A1 pAb tested by immunofluorescence. Staining of HeLa cells with Histone macroH2A1 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
IF (Immunofluorescence)
(Histone macroH2A1 pAb tested by immunofluorescence. Staining of HeLa cells with Histone macroH2A1 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Related Product Information for anti-macroH2A1 antibody
Short Description: Our Histone macroH2A1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.
Background: Histone macroH2A (mH2A) is a histone variant that has a region that is similar to histone H2A but has a unique C-terminal domain (the macro domain, also called the non-histone domain (NHD)) in addition to the histone-like region. mH2A associates with condensed chromatin, including the inactive mammalian female X chromosome, senescence-associated heterochromatin foci, imprinted genetic loci, and regions of chromatin that are CpG methylated. In vitro assays with mH2A have shown that nucleosomes containing macroH2A are resistant to ATP-dependent chromatin remodeling and transcription factor binding, and that macroH2A is capable of repressing the initiation of RNA pol II catalyzed transcription. MacroH2A1 consists of two isoforms, macroH2A1.1 and macroH2A1.2, that are produced by alternative splicing; they differ by only 30 amino acids in the C-terminal non-histone domain (NHD).
Background: Histone macroH2A (mH2A) is a histone variant that has a region that is similar to histone H2A but has a unique C-terminal domain (the macro domain, also called the non-histone domain (NHD)) in addition to the histone-like region. mH2A associates with condensed chromatin, including the inactive mammalian female X chromosome, senescence-associated heterochromatin foci, imprinted genetic loci, and regions of chromatin that are CpG methylated. In vitro assays with mH2A have shown that nucleosomes containing macroH2A are resistant to ATP-dependent chromatin remodeling and transcription factor binding, and that macroH2A is capable of repressing the initiation of RNA pol II catalyzed transcription. MacroH2A1 consists of two isoforms, macroH2A1.1 and macroH2A1.2, that are produced by alternative splicing; they differ by only 30 amino acids in the C-terminal non-histone domain (NHD).
Product Categories/Family for anti-macroH2A1 antibody
NCBI and Uniprot Product Information
Molecular Weight
41kDa
Similar Products
Product Notes
The macroH2A1 (Catalog #AAA59890) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone macroH2A1 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone macroH2A1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the macroH2A1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone macroH2A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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