IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human melanoma cancer using AAA117860 at dilution of 1:100)
Rabbit anti-Human Histone H3.3 Polyclonal Antibody | anti-H3.3 antibody
Rabbit anti-human Histone polyclonal Antibody
Gene Names
H3F3A; H3F3; H3.3A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Synonyms
Histone H3.3, Antibody; Rabbit anti-human Histone polyclonal Antibody; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; anti-H3.3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Sequence Length
136
Applicable Applications for anti-H3.3 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Histone H3.3 protein
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human melanoma cancer using AAA117860 at dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human melanoma cancer using AAA117860 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using AAA117860 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using AAA117860 at dilution of 1:100)
WB (Western Blot)
(Western blotAll lanes: Histone H3.3 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 15kDaObserved band size: 15kDa)
WB (Western Blot)
(Western blotAll lanes: Histone H3.3 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 15kDaObserved band size: 15kDa)
Related Product Information for anti-H3.3 antibody
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,328 Da
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone, family 3A
NCBI Official Symbol
H3F3A
NCBI Official Synonym Symbols
H3F3; H3.3A
NCBI Protein Information
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3F3A
UniProt Synonym Gene Names
H3.3A; H3F3
UniProt Entry Name
H33_HUMAN
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Product Notes
The H3.3 h3f3a (Catalog #AAA117860) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Histone polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3.3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the H3.3 h3f3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3.3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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