IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9(Phospho-Ser246/259/220) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Rabbit anti-Human HDAC4/HDAC5/HDAC9 Polyclonal Antibody | anti-HDAC4/HDAC5/HDAC9 antibody
HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody
Gene Names
HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
HDAC4/HDAC5/HDAC9, Antibody; HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody; HD4/HD5/HD9; anti-HDAC4/HDAC5/HDAC9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
1084
Applicable Applications for anti-HDAC4/HDAC5/HDAC9 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S(p)-EP) derived from Human HDAC4/HDAC5/HDAC9.
Modification
Phospho-Ser246/259/220
Target Name
HDAC4/HDAC5/HDAC9
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9(Phospho-Ser246/259/220) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9(Phospho-Ser246/259/220) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells and Hela cells treated with serum using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells and Hela cells treated with serum using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody.)
Related Product Information for anti-HDAC4/HDAC5/HDAC9 antibody
Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.
Product Categories/Family for anti-HDAC4/HDAC5/HDAC9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106,367 Da
NCBI Official Full Name
histone deacetylase 4
NCBI Official Synonym Full Names
histone deacetylase 4
NCBI Official Symbol
HDAC4
NCBI Official Synonym Symbols
HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
NCBI Protein Information
histone deacetylase 4
UniProt Protein Name
Histone deacetylase 4
UniProt Gene Name
HDAC4
UniProt Synonym Gene Names
KIAA0288; HD4
UniProt Entry Name
HDAC4_HUMAN
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Product Notes
The HDAC4/HDAC5/HDAC9 hdac4 (Catalog #AAA308059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC4/HDAC5/HDAC9 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HDAC4/HDAC5/HDAC9 hdac4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC4/HDAC5/HDAC9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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