FCM/FACS (Flow Cytometry)
(HBA2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit HBA2 Polyclonal Antibody | anti-HBA1 antibody
HBA2 Antibody (Center)
Gene Names
HBA2; HBH; HBA-T2
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HBA2, Antibody; HBA2 Antibody (Center); Hemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain; HBA1; anti-HBA1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HBA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the Central region of human HBA2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
100-128
Sequence Length
142
Applicable Applications for anti-HBA1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HBA2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HBA2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of HBA2 Antibody (Center) in mouse liver tissue lysates (35ug/lane). HBA2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of HBA2 Antibody (Center) in mouse liver tissue lysates (35ug/lane). HBA2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-HBA1 antibody
HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Product Categories/Family for anti-HBA1 antibody
References
Sessa, R., et al. Am. J. Hematol. 85(2):143-144(2010)
Sharma, V., et al. Hematology 14(5):297-300(2009)
Waye, J.S., et al. Hemoglobin 33(6):519-522(2009)
Roy, P., et al. Hemoglobin 33(6):486-491(2009)
Joly, P., et al. Hemoglobin 33(3):196-205(2009)
Sharma, V., et al. Hematology 14(5):297-300(2009)
Waye, J.S., et al. Hemoglobin 33(6):519-522(2009)
Roy, P., et al. Hemoglobin 33(6):486-491(2009)
Joly, P., et al. Hemoglobin 33(3):196-205(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15258
NCBI Official Full Name
hemoglobin subunit alpha
NCBI Official Synonym Full Names
hemoglobin, alpha 2
NCBI Official Symbol
HBA2
NCBI Official Synonym Symbols
HBH; HBA-T2
NCBI Protein Information
hemoglobin subunit alpha
UniProt Protein Name
Hemoglobin subunit alpha
UniProt Gene Name
HBA1
UniProt Entry Name
HBA_HUMAN
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Product Notes
The HBA1 hba1 (Catalog #AAA288835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 100-128. The HBA2 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's HBA2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HBA1 hba1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HBA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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