ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela(4^106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H3F3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
Rabbit anti-Human, Rat H3F3A Polyclonal Antibody | anti-H3F3A antibody
H3F3A(Ab-79) Antibody
Gene Names
H3F3A; H3F3; H3.3A
Reactivity
Human, Rat
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
H3F3A, Antibody; H3F3A(Ab-79) Antibody; Histone H3.3; H3F3A; H3.3A; H3F3; PP781; AND; H3F3B; H3.3B; anti-H3F3A antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
136
Applicable Applications for anti-H3F3A antibody
ChIP (Chromatin immunoprecipitation), WB (Western Blot), ELISA
Immunogen
Peptide sequence around site of Di-methyl-Lys(79) derived from human Histone H3.3
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela(4^106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H3F3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela(4^106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H3F3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat spleen tissueAll lanes: H3F3A antibody at 0.73ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 16 KDaObserved band size: 16 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat spleen tissueAll lanes: H3F3A antibody at 0.73ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 16 KDaObserved band size: 16 KDa)
Product Categories/Family for anti-H3F3A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,328 Da
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone family member 3A
NCBI Official Symbol
H3F3A
NCBI Official Synonym Symbols
H3F3; H3.3A
NCBI Protein Information
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3F3A
UniProt Synonym Gene Names
H3.3A; H3F3
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Product Notes
The H3F3A h3f3a (Catalog #AAA234734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H3F3A(Ab-79) Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's H3F3A can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the H3F3A h3f3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H3F3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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