IP (Immunoprecipitation)
(Detection of human GTF2E1/TFIIE-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody AAA211998 used for IP at 3 ug/mg lysate. GTF2E1/TFIIE-alpha was also immunoprecipitated by rabbit anti-GTF2E1/TFIIE-alpha antibody which recognizes a downstream epitope. For blotting immunoprecipitated GTF2E1/TFIIE-alpha, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human GTF2E1/TFIIE-alpha Polyclonal Antibody | anti-GTF2E1/TFIIE-alpha antibody
Rabbit anti-GTF2E1/TFIIE-alpha Antibody, Affinity Purified
Gene Names
GTF2E1; FE; TF2E1; TFIIE-A
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
GTF2E1/TFIIE-alpha, Antibody; Rabbit anti-GTF2E1/TFIIE-alpha Antibody, Affinity Purified; TFIIE alpha subunit; TFIIE-alpha; TFIIE-A; transcription initiation factor IIE subunit alpha; TF2E1; general transcription factor IIE, polypeptide 1, alpha 56kDa; general transcription factor IIE subunit 1; FE; general transcription factor IIE 56 kDa subunit; General transcription factor IIE subunit 1; anti-GTF2E1/TFIIE-alpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GTF2E1/TFIIE-alpha antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Bovine, Orangutan
Immunogen
Between 100 and 150
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human GTF2E1/TFIIE-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody AAA211998 used for IP at 3 ug/mg lysate. GTF2E1/TFIIE-alpha was also immunoprecipitated by rabbit anti-GTF2E1/TFIIE-alpha antibody which recognizes a downstream epitope. For blotting immunoprecipitated GTF2E1/TFIIE-alpha, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human GTF2E1/TFIIE-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody AAA211998 used for IP at 3 ug/mg lysate. GTF2E1/TFIIE-alpha was also immunoprecipitated by rabbit anti-GTF2E1/TFIIE-alpha antibody which recognizes a downstream epitope. For blotting immunoprecipitated GTF2E1/TFIIE-alpha, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human GTF2E1/TFIIE-alpha by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha (Cat. No. AAA211998 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human GTF2E1/TFIIE-alpha by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha (Cat. No. AAA211998 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-GTF2E1/TFIIE-alpha antibody
The general RNA polymerase transcription factor GTF2E1/TFIIE is composed of a heterodimer of an alpha and beta subunit. TFIIE has been shown to recruit TFIIH to promoters for the formation of the pre-initiation complex (PIC).
Product Categories/Family for anti-GTF2E1/TFIIE-alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,452 Da
NCBI Official Full Name
Homo sapiens general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mRNA
NCBI Official Synonym Full Names
general transcription factor IIE, polypeptide 1, alpha 56kDa
NCBI Official Symbol
GTF2E1
NCBI Official Synonym Symbols
FE; TF2E1; TFIIE-A
NCBI Protein Information
general transcription factor IIE subunit 1; TFIIE-alpha; general transcription factor IIE 56 kDa subunit; transcription initiation factor IIE subunit alpha
UniProt Protein Name
General transcription factor IIE subunit 1
UniProt Gene Name
GTF2E1
UniProt Synonym Gene Names
TF2E1; TFIIE-alpha
UniProt Entry Name
T2EA_HUMAN
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Product Notes
The GTF2E1/TFIIE-alpha gtf2e1 (Catalog #AAA211998) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GTF2E1/TFIIE-alpha Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2E1/TFIIE-alpha can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the GTF2E1/TFIIE-alpha gtf2e1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2E1/TFIIE-alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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