Rabbit GOLPH3 Polyclonal Antibody | anti-GOLPH3 antibody

GOLPH3 Antibody

Cat. Num. AAA40200

• Gene Names: GOLPH3; GOPP1; GPP34; MIDAS; Vps74
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, ELISA
• Purity: GOLPH3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: GOLPH3, Antibody; GOLPH3 Antibody; GOLPH3; GOPP1; GPP34; MIDAS; Golgi phosphoprotein 3; Coat protein GPP34; golgi phosphoprotein 3 (coat-protein); anti-GOLPH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: GOLPH3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 298

• Applicable Applications for anti-GOLPH3 antibody: IF (Immunofluorescence), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: GOLPH3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3.
• Buffer: GOLPH3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: GOLPH3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of GOLPH3 in Rat Lung cells with GOLPH3 antibody at 20 μg/mL.)

WB (Western Blot)

(Western blot analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-GOLPH3 antibody

GOLPH3 Antibody: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

NCBI and Uniprot Product Information

• NCBI GI #: 15082415
• NCBI GeneID: 64083
• UniProt Accession #: Q9H4A6; Q9UIW5
• Molecular Weight: 33,811 Da
• NCBI Official Full Name: Golgi phosphoprotein 3 (coat-protein)
• NCBI Official Synonym Full Names: golgi phosphoprotein 3 (coat-protein)
• NCBI Official Symbol: GOLPH3
• NCBI Official Synonym Symbols: GOPP1; GPP34; MIDAS; Vps74
• NCBI Protein Information: Golgi phosphoprotein 3; coat-protein; golgi protein; coat protein GPP34; golgi-associated protein; mitochondrial DNA absence factor; golgi peripheral membrane protein 1, 34 kDa
• UniProt Protein Name: Golgi phosphoprotein 3
• UniProt Gene Name: GOLPH3
• UniProt Synonym Gene Names: GPP34; MIDAS
• UniProt Entry Name: GOLP3_HUMAN