WB (Western Blot)
(Detection of human Golgin-160 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Golgin-160 antibody AAA212161 (lot AAA212161-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Golgin-160 Polyclonal Antibody | anti-GOLGA3 antibody
Rabbit anti-Golgin-160 Antibody, Affinity Purified
Gene Names
GOLGA3; MEA-2; GCP170
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Golgin-160, Antibody; Rabbit anti-Golgin-160 Antibody, Affinity Purified; golgin subfamily A member 3; MEA-2; male enhanced antigen-2; golgin-160; Golgi peripheral membrane protein; Golgi membrane associated protein; golgi complex-associated protein of 170 kDa; golgi autoantigen, golgin subfamily a, 3; GCP170; SY2/SY10 protein; golgin-165; Golgin subfamily A member 3; golgin A3; anti-GOLGA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GOLGA3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Golgin-160 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Golgin-160 antibody AAA212161 (lot AAA212161-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Golgin-160 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Golgin-160 antibody AAA212161 (lot AAA212161-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Golgin-160 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Golgin-160 antibody AAA212161 (lot AAA212161-1) used for IP at 6 ug per reaction. Golgin-160 was also immunoprecipitated by rabbit anti-Golgin-160 antibody For blotting immunoprecipitated Golgin-160, AAA212161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Golgin-160 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Golgin-160 antibody AAA212161 (lot AAA212161-1) used for IP at 6 ug per reaction. Golgin-160 was also immunoprecipitated by rabbit anti-Golgin-160 antibody For blotting immunoprecipitated Golgin-160, AAA212161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-GOLGA3 antibody
Golgin-160 belongs to the golgin family of proteins which localize to the Golgi. Golgin-160 has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport [taken from NCBI Entrez Gene (Gene ID: 2802)].
Product Categories/Family for anti-GOLGA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
167,355 Da
NCBI Official Full Name
Golgin subfamily A member 3 isoform 1
NCBI Official Synonym Full Names
golgin A3
NCBI Official Symbol
GOLGA3
NCBI Official Synonym Symbols
MEA-2; GCP170
NCBI Protein Information
Golgin subfamily A member 3; golgin-160; golgin-165; SY2/SY10 protein; male enhanced antigen-2; Golgi membrane associated protein; Golgi peripheral membrane protein; golgi autoantigen, golgin subfamily a, 3; golgi complex-associated protein of 170 kDa
UniProt Protein Name
Golgin subfamily A member 3
UniProt Gene Name
GOLGA3
UniProt Synonym Gene Names
GCP170
UniProt Entry Name
GOGA3_HUMAN
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Product Notes
The GOLGA3 golga3 (Catalog #AAA212161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Golgin-160 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Golgin-160 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GOLGA3 golga3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Golgin-160, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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