FOXA2 Polyclonal Antibody | anti-FOXA2 antibody

Goat Polyclonal to Human FOXA2

Cat. Num. AAA51048

• Gene Names: FOXA2; HNF3B; TCF3B
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FOXA2, Antibody; Goat Polyclonal to Human FOXA2; Anti-FOXA2 Antibody (C-Terminus) IHC-plus; FOXA2; HNF3B; HNF-3-beta; HNF-3B; TCF3B; Transcription factor 3B; Forkhead box A2; Forkhead box protein A2; Hepatic nuclear factor-3-beta; TCF-3B; Human FOXA2; anti-FOXA2 antibody

• Host: FOXA2 antibody was produced in Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
• Clonality: Polyclonal
• Specificity: Human FOZA2. This antibody is expected to recognise both reported isoforms
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-FOXA2 antibody: ELISA (Peptide ELISA), WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target: FOXA2
• Family: Transcription factor/ not assigned-transcription factor
• Immunogen Species: FOXA2 antibody was rasied against Human
• Immunogen: FOXA2 antibody was raised against peptide with sequence C_GVYSRPIMNSS, from C-Terminus of protein sequence according to NP_068556.NP_710141.1.
• Epitope: aa453-463
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

WB (Western Blot)

(Antibody staining (1 ug/ml) of Human Liver lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

IHC (Immunohistochemistry)

(Anti-FOXA2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Product Categories/Family for anti-FOXA2 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 194394143
• NCBI GeneID: 3170
• NCBI Accession #: NP_068556.2
• NCBI GenBank Nucleotide #: NM_021784.4
• UniProt Accession #: Q9Y261; Q8WUW4; Q96DF7
• NCBI Official Full Name: hepatocyte nuclear factor 3-beta isoform 1
• NCBI Official Synonym Full Names: forkhead box A2
• NCBI Official Symbol: FOXA2
• NCBI Official Synonym Symbols: HNF3B; TCF3B
• NCBI Protein Information: hepatocyte nuclear factor 3-beta; HNF-3B; TCF-3B; HNF-3-beta; forkhead box protein A2; transcription factor 3B; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta
• UniProt Protein Name: Hepatocyte nuclear factor 3-beta
• UniProt Gene Name: FOXA2
• UniProt Synonym Gene Names: HNF3B; TCF3B; HNF-3-beta; HNF-3B; TCF-3B
• UniProt Entry Name: FOXA2_HUMAN