WB (Western Blot)
(Detection of human and mouse FOXA1 by western blot. Samples: Whole cell lysate from MCF-7 (5, 15 and 50 ug), and HeLa (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FOXA1 antibody AAA213174 (lot AAA213174-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse FOXA1/HNF3A Polyclonal Antibody | anti-FOXA1/HNF3A antibody
Rabbit anti-FOXA1/HNF3A Antibody, Affinity Purified
Gene Names
FOXA1; HNF3A; TCF3A
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
FOXA1/HNF3A, Antibody; Rabbit anti-FOXA1/HNF3A Antibody, Affinity Purified; forkhead box protein A1; hepatocyte nuclear factor 3-alpha; HNF3A; HNF-3A; HNF-3-alpha; TCF3A; TCF-3A; transcription factor 3A; Hepatocyte nuclear factor 3-alpha; forkhead box A1; anti-FOXA1/HNF3A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FOXA1/HNF3A antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, X. laevis, X. tropicalis
Immunogen
Between 422 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse FOXA1 by western blot. Samples: Whole cell lysate from MCF-7 (5, 15 and 50 ug), and HeLa (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FOXA1 antibody AAA213174 (lot AAA213174-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse FOXA1 by western blot. Samples: Whole cell lysate from MCF-7 (5, 15 and 50 ug), and HeLa (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FOXA1 antibody AAA213174 (lot AAA213174-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human FOXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXA1 antibody AAA213174 (lot AAA213174-1) used for IP at 6 ug per reaction. FOXA1 was also immunoprecipitated by rabbit anti-FOXA1 antibody BL20490. For blotting immunoprecipitated FOXA1, AAA213174 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human FOXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXA1 antibody AAA213174 (lot AAA213174-1) used for IP at 6 ug per reaction. FOXA1 was also immunoprecipitated by rabbit anti-FOXA1 antibody BL20490. For blotting immunoprecipitated FOXA1, AAA213174 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-FOXA1/HNF3A antibody
Forkhead box protein A1 (FOXA1) is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver [taken from NCBI Entrez Gene (Gene ID: 3169)].
Product Categories/Family for anti-FOXA1/HNF3A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49148 MW
NCBI Official Full Name
hepatocyte nuclear factor 3-alpha
NCBI Official Synonym Full Names
forkhead box A1
NCBI Official Symbol
FOXA1
NCBI Official Synonym Symbols
HNF3A; TCF3A
NCBI Protein Information
hepatocyte nuclear factor 3-alpha
UniProt Protein Name
Hepatocyte nuclear factor 3-alpha
UniProt Gene Name
FOXA1
UniProt Synonym Gene Names
HNF3A; TCF3A; HNF-3-alpha; HNF-3A; TCF-3A
UniProt Entry Name
FOXA1_HUMAN
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Product Notes
The FOXA1/HNF3A foxa1 (Catalog #AAA213174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FOXA1/HNF3A Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FOXA1/HNF3A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FOXA1/HNF3A foxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXA1/HNF3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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