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Knockout Validation
(Knockout Validation: Lane 1: Wild-type RaW264.7 cell lysate;;Lane 2: FADD knockout RaW264.7 cell lysate;;Predicted MW: 23kDa ;Observed MW: 27kDa;Primary Ab: 1ug/ml Rabbit Anti-mouse FADD Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Mouse, Rat Fas Associating Death Domain Containing Protein (FADD) Polyclonal Antibody | anti-FADD antibody
Polyclonal Antibody to Fas Associating Death Domain Containing Protein (FADD)
Gene Names
Fadd; Mort1/FADD
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Antigen-specific Affinity Chromatography.
Synonyms
Fas Associating Death Domain Containing Protein (FADD), Antibody; Polyclonal Antibody to Fas Associating Death Domain Containing Protein (FADD); anti-FADD antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FADD. It has been selected for its ability to recognize FADD in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLHS LSGSLSGNDL MELKFLCRER VSKRKLERVQ SGLDLFTVLL EQNDLERGHT GLLRELLASL RRHDLLQRLD DFEAGTATAA PPGEADLQVA FDIVCDNVGR DWKRLARELK VSEAKMDGIE EKYPRSLSER VRESLKVWKN AEKKNASVAG LVKALRTCRL NLVADLVE
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLHS LSGSLSGNDL MELKFLCRER VSKRKLERVQ SGLDLFTVLL EQNDLERGHT GLLRELLASL RRHDLLQRLD DFEAGTATAA PPGEADLQVA FDIVCDNVGR DWKRLARELK VSEAKMDGIE EKYPRSLSER VRESLKVWKN AEKKNASVAG LVKALRTCRL NLVADLVE
Sequence Length
193
Applicable Applications for anti-FADD antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant Fas Associating Death Domain Containing Protein (FADD)
Cross Reactivity
Human
Source
Polyclonal antibody preparation
Traits
Liquid
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type RaW264.7 cell lysate;;Lane 2: FADD knockout RaW264.7 cell lysate;;Predicted MW: 23kDa ;Observed MW: 27kDa;Primary Ab: 1ug/ml Rabbit Anti-mouse FADD Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type RaW264.7 cell lysate;;Lane 2: FADD knockout RaW264.7 cell lysate;;Predicted MW: 23kDa ;Observed MW: 27kDa;Primary Ab: 1ug/ml Rabbit Anti-mouse FADD Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Human Serum.)
WB (Western Blot)
(Western Blot: Sample: Human Serum.)
Product Categories/Family for anti-FADD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,960 Da
NCBI Official Full Name
FAS-associated death domain protein
NCBI Official Synonym Full Names
Fas (TNFRSF6)-associated via death domain
NCBI Official Symbol
Fadd
NCBI Official Synonym Symbols
Mort1/FADD
NCBI Protein Information
FAS-associated death domain protein
UniProt Protein Name
FAS-associated death domain protein
UniProt Gene Name
Fadd
UniProt Synonym Gene Names
Mort1
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Product Notes
The FADD fadd (Catalog #AAA132153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Fas Associating Death Domain Containing Protein (FADD) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fas Associating Death Domain Containing Protein (FADD) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the FADD fadd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLHS LSGSLSGNDL MELKFLCRER VSKRKLERVQ SGLDLFTVLL EQNDLERGHT GLLRELLASL RRHDLLQRLD DFEAGTATAA PPGEADLQVA FDIVCDNVGR DWKRLARELK VSEAKMDGIE EKYPRSLSER VRESLKVWKN AEKKNASVAG LVKALRTCRL NLVADLVE. It is sometimes possible for the material contained within the vial of "Fas Associating Death Domain Containing Protein (FADD), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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