IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with EWSR1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.)
Rabbit EWSR1 Polyclonal Antibody | anti-EWSR1 antibody
EWSR1 Antibody (C-term)
Gene Names
EWSR1; EWS; EWS-FLI1; bK984G1.4
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EWSR1, Antibody; EWSR1 Antibody (C-term); RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein; EWSR1; EWS; anti-EWSR1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 623-652 amino acids from the C-terminal region of human EWSR1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
623-652
Sequence Length
655
Applicable Applications for anti-EWSR1 antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with EWSR1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with EWSR1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.)
WB (Western Blot)
(EWSR1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EWSR1 antibody detected the EWSR1 protein (arrow).)
WB (Western Blot)
(EWSR1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EWSR1 antibody detected the EWSR1 protein (arrow).)
Related Product Information for anti-EWSR1 antibody
This gene encodes a multifunctional protein that is
involved in various cellular processes, including gene expression,
cell signaling, and RNA processing and transport. The protein
includes an N-terminal transcriptional activation domain and a
C-terminal RNA-binding domain. Chromosomal translocations between
this gene and various genes encoding transcription factors result
in the production of chimeric proteins that are involved in
tumorigenesis. These chimeric proteins usually consist of the
N-terminal transcriptional activation domain of this protein fused
to the C-terminal DNA-binding domain of the transcription factor
protein. Mutations in this gene, specifically a t(11;22)(q24;q12)
translocation, are known to cause Ewing sarcoma as well as
neuroectodermal and various other tumors. Alternative splicing of
this gene results in multiple transcript variants. Related
pseudogenes have been identified on chromosomes 1 and 14. [provided
by RefSeq].
involved in various cellular processes, including gene expression,
cell signaling, and RNA processing and transport. The protein
includes an N-terminal transcriptional activation domain and a
C-terminal RNA-binding domain. Chromosomal translocations between
this gene and various genes encoding transcription factors result
in the production of chimeric proteins that are involved in
tumorigenesis. These chimeric proteins usually consist of the
N-terminal transcriptional activation domain of this protein fused
to the C-terminal DNA-binding domain of the transcription factor
protein. Mutations in this gene, specifically a t(11;22)(q24;q12)
translocation, are known to cause Ewing sarcoma as well as
neuroectodermal and various other tumors. Alternative splicing of
this gene results in multiple transcript variants. Related
pseudogenes have been identified on chromosomes 1 and 14. [provided
by RefSeq].
Product Categories/Family for anti-EWSR1 antibody
References
Lagirand-Cantaloube, J., et al. Biochem. Biophys. Res. Commun. 399(4):705-710(2010)
Kumagai, A., et al. Am. J. Clin. Pathol. 134(2):323-331(2010)
Aryee, D.N., et al. Cancer Res. 70(10):4015-4023(2010)
Riggi, N., et al. Genes Dev. 24(9):916-932(2010)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Kumagai, A., et al. Am. J. Clin. Pathol. 134(2):323-331(2010)
Aryee, D.N., et al. Cancer Res. 70(10):4015-4023(2010)
Riggi, N., et al. Genes Dev. 24(9):916-932(2010)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68478
NCBI Official Full Name
RNA-binding protein EWS isoform 3
NCBI Official Synonym Full Names
EWS RNA-binding protein 1
NCBI Official Symbol
EWSR1
NCBI Official Synonym Symbols
EWS; EWS-FLI1; bK984G1.4
NCBI Protein Information
RNA-binding protein EWS
UniProt Protein Name
RNA-binding protein EWS
UniProt Gene Name
EWSR1
UniProt Synonym Gene Names
EWS
UniProt Entry Name
EWS_HUMAN
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Product Notes
The EWSR1 ewsr1 (Catalog #AAA283983) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 623-652. The EWSR1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's EWSR1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the EWSR1 ewsr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EWSR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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