Rabbit EPHA4 Polyclonal Antibody | anti-EPHA4 antibody

EPHA4 Antibody

Cat. Num. AAA322122

• Gene Names: EPHA4; EK8; SEK; HEK8; TYRO1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: EPHA4, Antibody; EPHA4 Antibody; Cek 8; CEK8; EK8; eph receptor a4; EPH-like kinase 8; EPHA4; EPHA4_HUMAN; Ephrin type-A receptor 4; HEK 8; hEK8; Receptor protein-tyrosine kinase HEK8; Sek 1; SEK; TYRO 1 protein tyrosine kinase; TYRO1; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1; anti-EPHA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EPHA4 antibody detects endogenous levels of total EPHA4
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 986

• Applicable Applications for anti-EPHA4 antibody: ELISA, IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human EPHA4
• Subcellular Location: Membrane.
• Tissue Specificity: Ubiquitous.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(AAA322122 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot)

(Western blot analysis of EPHA4 expression in JurKat cells.The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-EPHA4 antibody

Function: Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
Subunit Structure: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts with CDK5, CDK5R1 and NGEF; upon activation by EFNA1 induces NGEF phosphorylation by the kinase CDK5. Interacts with CHN1; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation (By similarity). Interacts (via PDZ motif) with SIPA1L1 (via PDZ domain); controls neuronal morphology through regulation of the RAP1 (RAP1A or RAP1B) and RAP2 (RAP2A, RAP2B or RAP2C) GTPases.
Similarity: The protein kinase domain mediates interaction with NGEF. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 751557656
• NCBI GeneID: 2043
• NCBI Accession #: NP_001291465.1
• NCBI GenBank Nucleotide #: NM_001304536.1
• UniProt Accession #: P54764; Q2M380; A8K2P1; B2R601; B7Z6Q8
• Molecular Weight: Observed: 110 kD; Predicted: 110 kDa
• NCBI Official Full Name: ephrin type-A receptor 4 isoform a
• NCBI Official Synonym Full Names: EPH receptor A4
• NCBI Official Symbol: EPHA4
• NCBI Official Synonym Symbols: EK8; SEK; HEK8; TYRO1
• NCBI Protein Information: ephrin type-A receptor 4
• UniProt Protein Name: Ephrin type-A receptor 4
• UniProt Gene Name: EPHA4
• UniProt Synonym Gene Names: HEK8; SEK; TYRO1; EK8; hEK8