IHC (Immunohiostchemistry)
((2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat Dynactin Polyclonal Antibody | anti-DCTN1 antibody
Goat anti-Dynactin Antibody
Gene Names
DCTN1; P135; DP-150; DAP-150
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Dynactin, Antibody; Goat anti-Dynactin Antibody; DCTN1; dynactin 1 (p150, glued homolog, Drosophila); P135; DP-150; DAP-150; p150-glued; p150, Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150, Gl; DCTN1 antibody; dynactin 1 (p150; glued homolog; Drosophila) antibody; P135 antibody; DP-150 antibody; DAP-150 antibody; p150-glued antibody; p150; Glued (Drosophila) homolog antibody; 150 kDa dynein-associated polypeptide antibody; Glued (Drosophila) homolog) antibody; HMN7B antibody; OTTHUMP00000202491 antibody; dynactin 1 antibody; Glued homolog antibody; Dynactin; anti-DCTN1 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QEQLHQLHSRLIS
Sequence Length
1278
Applicable Applications for anti-DCTN1 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1.
Epitope
C Terminus
Note
This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IHC (Immunohiostchemistry)
((2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohiostchemistry)
((2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
WB (Western Blot)
(Staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
WB (Western Blot)
(Staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
References
Bupha-Intr T, Haizlip KM, Janssen PM.; Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.; Am J Physiol Heart Circ Physiol.2009 Mar;296(3): H806-14.; Pubmed 19136602
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
140,887 Da
NCBI Official Full Name
dynactin subunit 1 isoform 1
NCBI Official Synonym Full Names
dynactin subunit 1
NCBI Official Symbol
DCTN1
NCBI Official Synonym Symbols
P135; DP-150; DAP-150
NCBI Protein Information
dynactin subunit 1
UniProt Protein Name
Dynactin subunit 1
UniProt Gene Name
DCTN1
UniProt Synonym Gene Names
DP-150
UniProt Entry Name
DCTN1_HUMAN
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Product Notes
The DCTN1 dctn1 (Catalog #AAA60994) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Dynactin Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Dynactin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the DCTN1 dctn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QEQLHQLHSR LIS. It is sometimes possible for the material contained within the vial of "Dynactin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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