Rabbit DNM1L Polyclonal Antibody | anti-DNM1L antibody

DNM1L Antibody

Cat. Num. AAA301783

• Gene Names: DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: DNM1L, Antibody; DNM1L Antibody; DNM1L; DLP1; DRP1; DVLP; DYMPLE; anti-DNM1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DNM1L protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 725

• Applicable Applications for anti-DNM1L antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human DNM1L.
• Target Name: DNM1L
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cell using DNM1L antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded rat kidney using DNM1L antibody at dilution of 1:100 (200x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human esophageal cancer using DNM1L antibody at dilution of 1:100 (200x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using DNM1L antibody.)

Related Product Information for anti-DNM1L antibody

Dynamin-related protein 1 (DNM1L) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DNM1L affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DNM1L clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DNM1L at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DNM1L stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DNM1L is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DNM1L is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, and Huntington's disease (reviewed in 6).

Product Categories/Family for anti-DNM1L antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 510937022
• NCBI GeneID: 10059
• NCBI Accession #: NP_001265392.1
• NCBI GenBank Nucleotide #: NM_001278463.1
• UniProt Accession #: O00429; O14541; O60709; Q59GN9; Q7L6B3; Q8TBT7; Q9BWM1; Q9Y5J2; A8K4X9; B4DGC9; B4DSU8; J3KPI2
• Molecular Weight: 82,057 Da
• NCBI Official Full Name: dynamin-1-like protein isoform 4
• NCBI Official Synonym Full Names: dynamin 1-like
• NCBI Official Symbol: DNM1L
• NCBI Official Synonym Symbols: DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
• NCBI Protein Information: dynamin-1-like protein
• UniProt Protein Name: Dynamin-1-like protein
• UniProt Gene Name: DNM1L
• UniProt Synonym Gene Names: DLP1; DRP1; DVLP; Dymple; HdynIV
• UniProt Entry Name: DNM1L_HUMAN