WB (Western Blot)
(Detection of human DEC1 by western blot. Samples: Whole cell lysate (50 ug) from HDLM-2, GaMG, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DEC1 antibody (AAA211072 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human DEC1 Polyclonal Antibody | anti-DEC1 antibody
Rabbit anti-DEC1 Antibody, Affinity Purified
Gene Names
BHLHE40; DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DEC1, Antibody; Rabbit anti-DEC1 Antibody, Affinity Purified; class E basic helix-loop-helix protein 40; differentiated embryo chondrocyte expressed gene 1; Stra14; STRA13; stimulated by retinoic acid gene 13 protein; SHARP-2; SHARP2; HLHB2; enhancer-of-split and hairy-related protein 2; differentially expressed in chondrocytes protein 1; differentially expressed in chondrocytes 1; Clast5; class B basic helix-loop-helix protein 2; bHLHe40; BHLHB2; basic helix-loop-helix domain containing, class B, 2; DEC1; Class E basic helix-loop-helix protein 40; basic helix-loop-helix family member e40; anti-DEC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DEC1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 362 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DEC1 by western blot. Samples: Whole cell lysate (50 ug) from HDLM-2, GaMG, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DEC1 antibody (AAA211072 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human DEC1 by western blot. Samples: Whole cell lysate (50 ug) from HDLM-2, GaMG, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DEC1 antibody (AAA211072 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human DEC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DEC1 antibody (AAA211072 lot 3) used for IP at 6 ug per reaction. DEC1 was also immunoprecipitated by a second antibody against a different epitope of DEC1 (BL2927). For blotting immunoprecipitated DEC1, AAA211072 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human DEC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DEC1 antibody (AAA211072 lot 3) used for IP at 6 ug per reaction. DEC1 was also immunoprecipitated by a second antibody against a different epitope of DEC1 (BL2927). For blotting immunoprecipitated DEC1, AAA211072 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry)
(Detection of human DEC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DEC1 (Cat. No. AAA211072 Lot3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DEC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DEC1 (Cat. No. AAA211072 Lot3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-DEC1 antibody
DEC1 is a basic helix-loop-helix transcription factor that appears to play a role in cell differentiation, proliferation, apoptosis, and the circadian rhythm. DEC1 is able to promote chondrogenic and osteogenic differentiation in mesenchymal stem cells by activating transcription of bone-related genes. When it is overexpressed in cells it can suppress apoptosis by blocking caspase activation and inducing transcription of survivin. DEC1 has also been found to play a role mammalian in circadian rhythm as a suppressor of the CLOCK/BMAL1 promoter.
Product Categories/Family for anti-DEC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,510 Da
NCBI Official Full Name
class E basic helix-loop-helix protein 40
NCBI Official Synonym Full Names
basic helix-loop-helix family, member e40
NCBI Official Symbol
BHLHE40
NCBI Official Synonym Symbols
DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
NCBI Protein Information
class E basic helix-loop-helix protein 40; class B basic helix-loop-helix protein 2; differentially expressed in chondrocytes 1; stimulated by retinoic acid gene 13 protein; enhancer-of-split and hairy-related protein 2; differentially expressed in chondr
UniProt Protein Name
Class E basic helix-loop-helix protein 40
UniProt Gene Name
BHLHE40
UniProt Synonym Gene Names
BHLHB2; DEC1; SHARP2; STRA13; bHLHe40; bHLHb2; DEC1; SHARP-2
UniProt Entry Name
BHE40_HUMAN
Similar Products
Product Notes
The DEC1 bhlhe40 (Catalog #AAA211072) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DEC1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DEC1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DEC1 bhlhe40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.