Rabbit anti-Human, mouse DDC Polyclonal Antibody | anti-DDC antibody

DDC Antibody (N-term)

Cat. Num. AAA284110

• Gene Names: DDC; AADC
• Reactivity: Human, mouse
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DDC, Antibody; DDC Antibody (N-term); Aromatic-L-amino-acid decarboxylase; AADC; DOPA decarboxylase; DDC; anti-DDC antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This DDC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human DDC.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 32-61
• Sequence Length: 480

• Applicable Applications for anti-DDC antibody: WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
• Function: Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, and L-5-hydroxytryptophan to serotonin.
• Tissue Location: [Isoform 2]: High expression in kidney.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(DDC Antibody (N-term) (Cat. # AAA284110) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(DDC Antibody (N-term) (Cat. # AAA284110) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Anti-DDC Antibody (N-term) at 1:1000 dilution + human kidney lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(DDC Antibody (N-term) (Cat. # AAA284110) western blot analysis in mouse liver,kidney tissue lysates (15ug/lane).This demonstrates the DDC antibody detected DDC protein (arrow).)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of DDC Antibody (N-term)(AAA284110) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-DDC antibody

This protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency.

Product Categories/Family for anti-DDC antibody

Cancer; Metabolism; Neuroscience

References

Kokkinou, I., et al. J. Neuroimmunol. 216 (1-2), 51-58 (2009) : Meda, S.A., et al. Neuroimage (2009) Ishikawa, S., et al. J. Biol. Chem. 284(42):28832-28844(2009) Trevino, L.R., et al. Nat. Genet. 41(9):1001-1005(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 4503281
• NCBI GeneID: 1644
• NCBI Accession #: NP_000781.1; NP_001076440.1; NP_001229815.1; NP_001229816.1; NP_001229817.1; NP_001229818.1; NP_001229819.1
• NCBI GenBank Nucleotide #: NM_000790.3
• UniProt Accession #: P20711; Q16723; Q5W5T9; Q75MJ6; C9IYA0; E7ER62; E7EU95
• NCBI Official Full Name: aromatic-L-amino-acid decarboxylase isoform 1
• NCBI Official Synonym Full Names: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• NCBI Official Symbol: DDC
• NCBI Official Synonym Symbols: AADC
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase
• UniProt Protein Name: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: DDC
• UniProt Synonym Gene Names: AADC; AADC; DDC
• UniProt Entry Name: DDC_HUMAN