Rabbit anti-Human, Mouse CRYAB Polyclonal Antibody | anti-CRYAB antibody

CRYAB Antibody

Cat. Num. AAA303462

• Gene Names: CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification.
• Synonyms: CRYAB, Antibody; CRYAB Antibody; CRYA2; CTPP2; HSPB5; CMD1II; anti-CRYAB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total CRYAB protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 175

• Applicable Applications for anti-CRYAB antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Full length fusion protein
• Target Name: CRYAB
• Preparation and Storage: Store at -20 degree C

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/25.)

Application Data

(Gel: 12%SDS-PAGE Lysates (from left to right): Mouse heart and human chromaffin cell tumor tissue, mouse muscle tissue Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

Related Product Information for anti-CRYAB antibody

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Product Categories/Family for anti-CRYAB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 577019571
• NCBI GeneID: 1410
• NCBI Accession #: NP_001276736.1
• NCBI GenBank Nucleotide #: NM_001289807.1
• UniProt Accession #: P02511; O43416; Q9UC37; Q9UC38; Q9UC39; Q9UC40; Q9UC41; B0YIX0
• Molecular Weight: 20,159 Da
• NCBI Official Full Name: alpha-crystallin B chain
• NCBI Official Synonym Full Names: crystallin, alpha B
• NCBI Official Symbol: CRYAB
• NCBI Official Synonym Symbols: MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• NCBI Protein Information: alpha-crystallin B chain
• UniProt Protein Name: Alpha-crystallin B chain
• UniProt Gene Name: CRYAB
• UniProt Synonym Gene Names: CRYA2; HspB5
• UniProt Entry Name: CRYAB_HUMAN