Rabbit CIITA Polyclonal Antibody | anti-CIITA antibody

Anti-CIITA Antibody

Cat. Num. AAA46642

• Gene Names: CIITA; C2TA; NLRA; MHC2TA; CIITAIV
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: CIITA, Antibody; Anti-CIITA Antibody; C2TA; CIITA; CIITA IV; MHC2TA; NLRA; P33076; MHC class II transactivator; class II, major histocompatibility complex, transactivator; anti-CIITA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 1130

• Applicable Applications for anti-CIITA antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived human CIITA recombinant protein (Position: E945-R1130). Human CIITA shares 84.4% amino acid (aa) sequence identity with mouse CIITA.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(CIITA was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemisry)

(CIITA was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohiostchemistry)

(CIITA was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot)

(Western blot analysis of CIITA expression in rat thymus extract (lane 1), mouse thymus extract (lane 2) and MCF-7 whole cell lysates (lane 3). CIITA at 123KD was detected using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

Related Product Information for anti-CIITA antibody

Rabbit IgG polyclonal antibody for MHC class II transactivator (CIITA) detection.
Background: CIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.

References

1. Raval A, Howcroft TK, Weissman JD, Kirshner S, Zhu XS, Yokoyama K, Ting J, Singer DS (Jan 2001). "Transcriptional coactivator, CIITA, is an acetyltransferase that bypasses a promoter requirement for TAF(II)250".Molecular Cell 7 (1): 105-15.
2. Steidl C, Shah SP, Woolcock BW, Rui L, Kawahara M, Farinha P, Johnson NA, Zhao Y, Telenius A, Neriah SB, McPherson A, Meissner B, Okoye UC, Diepstra A, van den Berg A, Sun M, Leung G, Jones SJ, Connors JM, Huntsman DG, Savage KJ, Rimsza LM, Horsman DE, Staudt LM, Steidl U, Marra MA, Gascoyne RD (Mar 2011). "MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers". Nature 471 (7338): 377-81.

NCBI and Uniprot Product Information

• NCBI GI #: 156938336
• NCBI GeneID: 4261
• NCBI Accession #: NP_000237.2
• NCBI GenBank Nucleotide #: NM_000246.3
• UniProt Accession #: P33076; Q29675; Q8SNB8; Q96KL4; A0N0N9; D3DUG0; E9PFE0
• NCBI Official Full Name: MHC class II transactivator isoform 2
• NCBI Official Synonym Full Names: class II, major histocompatibility complex, transactivator
• NCBI Official Symbol: CIITA
• NCBI Official Synonym Symbols: C2TA; NLRA; MHC2TA; CIITAIV
• NCBI Protein Information: MHC class II transactivator
• UniProt Protein Name: MHC class II transactivator
• UniProt Gene Name: CIITA
• UniProt Synonym Gene Names: MHC2TA; CIITA
• UniProt Entry Name: C2TA_HUMAN