IF (Immunofluorescence)
(AAA320983 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF0349) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Rabbit CIB2 Polyclonal Antibody | anti-CIB2 antibody
CIB2 Antibody
Gene Names
CIB2; KIP2; USH1J; DFNB48
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
CIB2, Antibody; CIB2 Antibody; Calcium and integrin binding protein 2; Calcium and integrin-binding family member 2; Cib2; CIB2_HUMAN; DNA dependent protein kinase interacting protein 2; Kinase interacting protein 2; Kinase-interacting protein 2; KIP 2; anti-CIB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CIB2 antibody detects endogenous levels of CIB2
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
144
Applicable Applications for anti-CIB2 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CIB2 corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA320983 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF0349) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA320983 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF0349) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain tissue, using CIB2 Antibody. The lane on the left was treated with blocking peptide..)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain tissue, using CIB2 Antibody. The lane on the left was treated with blocking peptide..)
IHC (Immunohistochemistry)
(AAA320983 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA320983 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CIB2 antibody
CIB Protein type : Calcium-binding protein Molecular Function: calcium ion binding.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21 kDa
NCBI Official Full Name
calcium and integrin-binding family member 2 isoform 2
NCBI Official Synonym Full Names
calcium and integrin binding family member 2
NCBI Official Symbol
CIB2
NCBI Official Synonym Symbols
KIP2; USH1J; DFNB48
NCBI Protein Information
calcium and integrin-binding family member 2
UniProt Protein Name
Calcium and integrin-binding family member 2
UniProt Gene Name
CIB2
UniProt Synonym Gene Names
KIP2; KIP 2
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Product Notes
The CIB2 cib2 (Catalog #AAA320983) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CIB2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CIB2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CIB2 cib2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CIB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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