Rabbit anti-Human, Mouse Ceramide Kinase / CERK Polyclonal Antibody | anti-CERK antibody

Rabbit Polyclonal to Human Ceramide Kinase / CERK

Cat. Num. AAA52431

• Gene Names: CERK; LK4; hCERK; dA59H18.2; dA59H18.3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Ammonium sulfate precipitation
• Synonyms: Ceramide Kinase / CERK, Antibody; Rabbit Polyclonal to Human Ceramide Kinase / CERK; CERK , Acylsphingosine kinase, Ceramide kinase, DA59H18.2, DA59H18.3, KIAA1646, Lipid kinase 4, Lipid kinase LK4, HCERK, LK4; anti-CERK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: This CERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-516 amino acids from the C-terminal region of human CERK.
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 537

• Applicable Applications for anti-CERK antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Family/Subfamily: Non-Protein Kinase/ Sphingosine
• Immunogen: Ceramide Kinase / CERK antibody was raised against human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

WB (Western Blot)

(The anti-CERK antibody is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).)

IHC (Immunohistochemistry)

(Anti-Ceramide Kinase / CERK antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Product Categories/Family for anti-CERK antibody

Family: Non-Protein Kinase; Subfamily: Sphingosine

NCBI and Uniprot Product Information

• NCBI GI #: 20336726
• NCBI GeneID: 64781
• NCBI Accession #: NP_073603.2
• NCBI GenBank Nucleotide #: NM_022766.5
• UniProt Accession #: Q8TCT0; Q6NX59; Q9BYB3; Q9UGE5; A0JNT4; A8K611
• NCBI Official Full Name: ceramide kinase
• NCBI Official Synonym Full Names: ceramide kinase
• NCBI Official Symbol: CERK
• NCBI Official Synonym Symbols: LK4; hCERK; dA59H18.2; dA59H18.3
• NCBI Protein Information: ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase
• UniProt Protein Name: Ceramide kinase
• UniProt Gene Name: CERK
• UniProt Synonym Gene Names: KIAA1646; hCERK; LK4
• UniProt Entry Name: CERK1_HUMAN