Rabbit anti-Mouse CD3 epsilon/CD3e Polyclonal Antibody | anti-CD3E antibody

Anti-CD3 epsilon/CD3e Antibody, Rabbit Polyclonal

Cat. Num. AAA255228

• Gene Names: Cd3e; CD3; T3e; AI504783; CD3epsilon
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CD3 epsilon/CD3e, Antibody; Anti-CD3 epsilon/CD3e Antibody, Rabbit Polyclonal; CD3 epsilon/CD3e Antibody, Rabbit PAb, Antigen Affinity Purified; CD3e molecule, epsilon (CD3-TCR complex); Anti-AI504783 Antibody; Anti-CD3 Antibody; Anti-CD3epsilon Antibody; Anti-T3e Antibody; anti-CD3E antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CD3 epsilon/CD3e
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CD3E antibody: IHC (Immunohistochemistry)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Mouse CD3 epsilon/CD3e
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse CD3 epsilon/CD3e, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Immunochemical staining of mouse CD3 in mouse small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining of mouse CD3 in mouse spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-CD3E antibody

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naive T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

Product Categories/Family for anti-CD3E antibody

ITIM/ITAM Immunoreceptors and Related Molecules; T Cell Receptor Signaling Pathway

References

Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.

NCBI and Uniprot Product Information

• NCBI GI #: 6680886
• NCBI GeneID: 12501
• NCBI Accession #: NP_031674.1
• NCBI GenBank Nucleotide #: NM_007648.4
• UniProt Accession #: P22646
• Molecular Weight: 21,393 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3 antigen, epsilon polypeptide
• NCBI Official Symbol: Cd3e
• NCBI Official Synonym Symbols: CD3; T3e; AI504783; CD3epsilon
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: Cd3e
• UniProt Entry Name: CD3E_MOUSE