IHC (Immunohistochemisry)
(CD130 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CD130 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human CD130 Polyclonal Antibody | anti-IL6ST antibody
CD130 Antibody (C-term)
Gene Names
IL6ST; CD130; GP130; CDW130; IL-6RB
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD130, Antibody; CD130 Antibody (C-term); Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; CDw130; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; IL6ST; anti-IL6ST antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD130 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 871-899 amino acids from the C-terminal region of human CD130.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.25 (varies by lot)
Sequence Positions
871-899
Sequence Length
857
Applicable Applications for anti-IL6ST antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(CD130 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CD130 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(CD130 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CD130 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(CD130 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CD130 antibody detected the CD130 protein (arrow).)
WB (Western Blot)
(CD130 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CD130 antibody detected the CD130 protein (arrow).)
WB (Western Blot)
(Anti-CD130 Antibody (C-term)at 1:2000 dilution + SW480 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CD130 Antibody (C-term)at 1:2000 dilution + SW480 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-IL6ST antibody
The protein encoded by this gene is a signal transducer
shared by many cytokines, including interleukin 6 (IL6), ciliary
neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and
oncostatin M (OSM). This protein functions as a part of the
cytokine receptor complex. The activation of this protein is
dependent upon the binding of cytokines to their receptors. vIL6, a
protein related to IL6 and encoded by the Kaposi sarcoma-associated
herpesvirus, can bypass the interleukin 6 receptor (IL6R) and
directly activate this protein. Knockout studies in mice suggest
that this gene plays a critical role in regulating myocyte
apoptosis. Alternatively spliced transcript variants encoding
distinct isoforms have been described. A related pseudogene has
been identified on chromosome 17.
shared by many cytokines, including interleukin 6 (IL6), ciliary
neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and
oncostatin M (OSM). This protein functions as a part of the
cytokine receptor complex. The activation of this protein is
dependent upon the binding of cytokines to their receptors. vIL6, a
protein related to IL6 and encoded by the Kaposi sarcoma-associated
herpesvirus, can bypass the interleukin 6 receptor (IL6R) and
directly activate this protein. Knockout studies in mice suggest
that this gene plays a critical role in regulating myocyte
apoptosis. Alternatively spliced transcript variants encoding
distinct isoforms have been described. A related pseudogene has
been identified on chromosome 17.
Product Categories/Family for anti-IL6ST antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Goette, N.P., et al. Exp. Hematol. 38(10):868-876(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Richards, P.J., et al. Arthritis Rheum. 54(5):1662-1672(2006)
Goette, N.P., et al. Exp. Hematol. 38(10):868-876(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Richards, P.J., et al. Arthritis Rheum. 54(5):1662-1672(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103537
NCBI Official Full Name
interleukin-6 receptor subunit beta isoform 3
NCBI Official Synonym Full Names
interleukin 6 signal transducer
NCBI Official Symbol
IL6ST
NCBI Official Synonym Symbols
CD130; GP130; CDW130; IL-6RB
NCBI Protein Information
interleukin-6 receptor subunit beta
UniProt Protein Name
Interleukin-6 receptor subunit beta
UniProt Gene Name
IL6ST
UniProt Synonym Gene Names
IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130
UniProt Entry Name
IL6RB_HUMAN
Similar Products
Product Notes
The IL6ST il6st (Catalog #AAA289373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 871-899. The CD130 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD130 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IL6ST il6st for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD130, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.