FCM/FACS (Flow Cytometry)
(CALR Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse CALR Polyclonal Antibody | anti-CALR antibody
CALR Antibody
Gene Names
CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CALR, Antibody; CALR Antibody; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60; CALR; CRTC; anti-CALR antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CALR Antibody is generated from rabbits immunized with a recombinant protein of human CALR.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
417
Applicable Applications for anti-CALR antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CALR Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CALR Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(CALR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CALR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(CALR Antibody western blot analysis in Hela,SH-SY5Y,rat C6 cell line lysates (35ug/lane).This demonstrates the CALR antibody detected the CALR protein (arrow).)
WB (Western Blot)
(CALR Antibody western blot analysis in Hela,SH-SY5Y,rat C6 cell line lysates (35ug/lane).This demonstrates the CALR antibody detected the CALR protein (arrow).)
Related Product Information for anti-CALR antibody
Calreticulin is a multifunctional protein that acts as a
major Ca(2+)-binding (storage) protein in the lumen of the
endoplasmic reticulum. It is also found in the nucleus, suggesting
that it may have a role in transcription regulation. Calreticulin
binds to the synthetic peptide KLGFFKR, which is almost identical
to an amino acid sequence in the DNA-binding domain of the
superfamily of nuclear receptors. Calreticulin binds to antibodies
in certain sera of systemic lupus and Sjogren patients which
contain anti-Ro/SSA antibodies, it is highly conserved among
species, and it is located in the endoplasmic and sarcoplasmic
reticulum where it may bind calcium. The amino terminus of
calreticulin interacts with the DNA-binding domain of the
glucocorticoid receptor and prevents the receptor from binding to
its specific glucocorticoid response element. Calreticulin can
inhibit the binding of androgen receptor to its hormone-responsive
DNA element and can inhibit androgen receptor and retinoic acid
receptor transcriptional activities in vivo, as well as retinoic
acid-induced neuronal differentiation. Thus, calreticulin can act
as an important modulator of the regulation of gene transcription
by nuclear hormone receptors. Systemic lupus erythematosus is
associated with increased autoantibody titers against calreticulin
but calreticulin is not a Ro/SS-A antigen. Earlier papers referred
to calreticulin as an Ro/SS-A antigen but this was later disproven.
Increased autoantibody titer against human calreticulin is found in
infants with complete congenital heart block of both the IgG and
IgM classes.
major Ca(2+)-binding (storage) protein in the lumen of the
endoplasmic reticulum. It is also found in the nucleus, suggesting
that it may have a role in transcription regulation. Calreticulin
binds to the synthetic peptide KLGFFKR, which is almost identical
to an amino acid sequence in the DNA-binding domain of the
superfamily of nuclear receptors. Calreticulin binds to antibodies
in certain sera of systemic lupus and Sjogren patients which
contain anti-Ro/SSA antibodies, it is highly conserved among
species, and it is located in the endoplasmic and sarcoplasmic
reticulum where it may bind calcium. The amino terminus of
calreticulin interacts with the DNA-binding domain of the
glucocorticoid receptor and prevents the receptor from binding to
its specific glucocorticoid response element. Calreticulin can
inhibit the binding of androgen receptor to its hormone-responsive
DNA element and can inhibit androgen receptor and retinoic acid
receptor transcriptional activities in vivo, as well as retinoic
acid-induced neuronal differentiation. Thus, calreticulin can act
as an important modulator of the regulation of gene transcription
by nuclear hormone receptors. Systemic lupus erythematosus is
associated with increased autoantibody titers against calreticulin
but calreticulin is not a Ro/SS-A antigen. Earlier papers referred
to calreticulin as an Ro/SS-A antigen but this was later disproven.
Increased autoantibody titer against human calreticulin is found in
infants with complete congenital heart block of both the IgG and
IgM classes.
Product Categories/Family for anti-CALR antibody
References
Du, X.L., et al. Oncogene 28(42):3714-3722(2009)
Sato, H., et al. Biochim. Biophys. Acta 1790(10):1198-1205(2009)
Abd Alla, J., et al. Biochem. Biophys. Res. Commun. 387(1):186-190(2009)
Sato, H., et al. Biochim. Biophys. Acta 1790(10):1198-1205(2009)
Abd Alla, J., et al. Biochem. Biophys. Res. Commun. 387(1):186-190(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48142
NCBI Official Full Name
calreticulin
NCBI Official Synonym Full Names
calreticulin
NCBI Official Symbol
CALR
NCBI Official Synonym Symbols
RO; CRT; SSA; cC1qR; HEL-S-99n
NCBI Protein Information
calreticulin
UniProt Protein Name
Calreticulin
UniProt Gene Name
CALR
UniProt Synonym Gene Names
CRTC; ERp60
UniProt Entry Name
CALR_HUMAN
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Product Notes
The CALR calr (Catalog #AAA290032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CALR Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CALR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CALR calr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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