IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/40.)
Rabbit anti-Human, Mouse BRK1 Polyclonal Antibody | anti-BRK1 antibody
BRK1 Antibody
Gene Names
BRK1; MDS027; hHBrk1; C3orf10; HSPC300
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification.
Synonyms
BRK1, Antibody; BRK1 Antibody; MDS027; hHBrk1; C3orf10; HSPC300; anti-BRK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total BRK1 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
2.6 mg/ml (varies by lot)
Sequence Length
75
Applicable Applications for anti-BRK1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to residues near the C terminal of human BRICK1, SCAR/WAVE actin-nucleating complex subunit
Target Name
BRK1
Preparation and Storage
Store at -20 degree C
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/40.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/40.)
Application Data
(Gel: 10%SDS-PAGE Lysate: 40ug A172 cell Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Application Data
(Gel: 10%SDS-PAGE Lysate: 40ug A172 cell Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Related Product Information for anti-BRK1 antibody
HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms.
Product Categories/Family for anti-BRK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
SDS-PAGE MW: 9kd
NCBI Official Full Name
protein BRICK1
NCBI Official Synonym Full Names
BRICK1, SCAR/WAVE actin-nucleating complex subunit
NCBI Official Symbol
BRK1
NCBI Official Synonym Symbols
MDS027; hHBrk1; C3orf10; HSPC300
NCBI Protein Information
protein BRICK1
UniProt Protein Name
Protein BRICK1
UniProt Gene Name
BRK1
UniProt Synonym Gene Names
C3orf10; BRK1
UniProt Entry Name
BRK1_HUMAN
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Product Notes
The BRK1 brk1 (Catalog #AAA305157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRK1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BRK1 brk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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