WB (Western Blot)
(Detection of human BRCA2 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, HeLa, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA2 antibody AAA212166 (lot AAA212166-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human BRCA2 Polyclonal Antibody | anti-BRCA2 antibody
Rabbit anti-BRCA2 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BRCA2, Antibody; Rabbit anti-BRCA2 Antibody, Affinity Purified; FAD1; FANCD; Fanconi anemia group D1 protein; mutant BRCA2; PNCA2; XRCC11; FANCD1; FAD; truncated breast cancer 2; BROVCA2; breast cancer type 2 susceptibility protein; BRCA1/BRCA2-containing complex, subunit 2; breast cancer 2, early onset; GLM3; breast cancer 2 tumor suppressor; breast and ovarian cancer susceptibility protein 2; breast and ovarian cancer susceptibility gene, early onset; BRCC2; FACD; Breast cancer type 2 susceptibility protein; BRCA2, DNA repair associated; anti-BRCA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BRCA2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 450 and 500
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human BRCA2 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, HeLa, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA2 antibody AAA212166 (lot AAA212166-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human BRCA2 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, HeLa, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA2 antibody AAA212166 (lot AAA212166-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRCA2 antibody AAA212166 (lot AAA212166-3) used for IP at 6 ug per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot AAA212166-2). For blotting immunoprecipitated BRCA2, AAA212166 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRCA2 antibody AAA212166 (lot AAA212166-3) used for IP at 6 ug per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot AAA212166-2). For blotting immunoprecipitated BRCA2, AAA212166 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-BRCA2 antibody
BRCA2 was identified as a gene with germline mutations that predisposes women to breast cancer. An important function of BRCA2 involves its direct interaction with RAD51 and the process of homologous recombination in DNA double strand break repair. BRCA2 and BRCA1 primary sequences are not similar; however the proteins exhibit similar patterns of expression and sub-cellular localization. Although the phenotype of disruptions of BRCA2 is similar to BRCA1, BRCA2 appears to possess functions distinct from BRCA1.
Product Categories/Family for anti-BRCA2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The BRCA2 brca2 (Catalog #AAA212166) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRCA2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRCA2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BRCA2 brca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRCA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.