Rabbit anti-Human BMPR2 Polyclonal Antibody | anti-BMPR2 antibody

BMPR2 Antibody (N-term)

Cat. Num. AAA288640

• Gene Names: BMPR2; BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
• Synonyms: BMPR2, Antibody; BMPR2 Antibody (N-term); Bone morphogenetic protein receptor type-2; BMP type-2 receptor; BMPR-2; Bone morphogenetic protein receptor type II; BMP type II receptor; BMPR-II; BMPR2; PPH1; anti-BMPR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This BMPR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-59 amino acids from the N-terminal region of human BMPR2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 28-59
• Sequence Length: 1038

• Applicable Applications for anti-BMPR2 antibody: WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
• Function: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Can also mediate signaling through the activation of the p38MAPK cascade (PubMed:12045205). Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6.
• Cellular Location: Cell membrane; Single-pass type I membrane protein
• Tissue Location: Highly expressed in heart and liver.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Anti-BMPR2 Antibody (D43) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of HepG2 cells using BMPR2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human Kidney tissue reacted with BMPR2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with BMPR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of anti-BMPR2 Pab in mouse heart tissue lysate. BMPR2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-BMPR2 antibody

BMPR2 is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in BMPR2 have been associated with primary pulmonary hypertension.

Product Categories/Family for anti-BMPR2 antibody

Cardiovascular; Signal Transduction

References

Pouliot, F., et al., Cancer Res. 63(2):277-281 (2003). Nishihara, A., et al., Mol. Biol. Cell 13(9):3055-3063 (2002). Humbert, M., et al., Eur Respir J 20(3):518-523 (2002). Vitt, U.A., et al., Biol. Reprod. 67(2):473-480 (2002). Nohe, A., et al., J. Biol. Chem. 277(7):5330-5338 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 15451916
• NCBI GeneID: 659
• NCBI Accession #: NP_001195.2
• NCBI GenBank Nucleotide #: NM_001204.6
• UniProt Accession #: Q13873; Q13161; Q16569; Q4ZG08; Q53SA5; Q585T8
• NCBI Official Full Name: bone morphogenetic protein receptor type-2
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type II (serine/threonine kinase)
• NCBI Official Symbol: BMPR2
• NCBI Official Synonym Symbols: BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
• NCBI Protein Information: bone morphogenetic protein receptor type-2
• UniProt Protein Name: Bone morphogenetic protein receptor type-2
• UniProt Gene Name: BMPR2
• UniProt Synonym Gene Names: PPH1; BMP type-2 receptor; BMPR-2; BMP type II receptor; BMPR-II
• UniProt Entry Name: BMPR2_HUMAN