WB (Western Blot)
(Western blot analysis using AMPK1/AMPK2 (Phospho-Ser485/Ser491) antibody.Lane 1: The extracts from HeLa cells; Lane 2: The extracts from HeLa cells treated with heat shock)
Rabbit AMPK Polyclonal Antibody | anti-AMPK antibody
AMPK antibody (Ser485/Ser491)
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
AMPK, Antibody; AMPK antibody (Ser485/Ser491); Polyclonal AMPK; Anti-AMPK; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase; catalytic alpha-2 chain; AAPK2; AMPK; AMPK subunit alpha-2; AMPK-alpha-2 chain; AMPK2; PRKAA; PRKAA2; protein kinase; AMP-activated; alpha 2 catalytic subunit; anti-AMPK antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
AMPK Antibody detects endogenous levels of AMPK only when phosphorylated at Serine 485 or Serine 491
Form/Format
Supplied in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
552
Applicable Applications for anti-AMPK antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Biological Significance
AMPK is a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism.
Cross-Reactivity
Mouse, Rat
Immunogen
AMPK antibody was raised in Rabbit using synthesized phosphopeptide derived from human AMPK around the phosphorylation site of Serine 485/491as the immunogen
Preparation and Storage
Store at -20 degree C for 1 year
WB (Western Blot)
(Western blot analysis using AMPK1/AMPK2 (Phospho-Ser485/Ser491) antibody.Lane 1: The extracts from HeLa cells; Lane 2: The extracts from HeLa cells treated with heat shock)
WB (Western Blot)
(Western blot analysis using AMPK1/AMPK2 (Phospho-Ser485/Ser491) antibody.Lane 1: The extracts from HeLa cells; Lane 2: The extracts from HeLa cells treated with heat shock)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded colon carcinoma, using AMPK1/AMPK2 (Phospho-Ser485/Ser491) antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded colon carcinoma, using AMPK1/AMPK2 (Phospho-Ser485/Ser491) antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide)
Related Product Information for anti-AMPK antibody
Purified Rabbit polyclonal AMPK antibody (Ser485/Ser491)
Product Categories/Family for anti-AMPK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,320 Da
NCBI Official Full Name
AMP-activated protein kinase, AMPK
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 2 catalytic subunit
NCBI Official Symbol
PRKAA2
NCBI Official Synonym Symbols
AMPK; AMPK2; PRKAA; AMPKa2
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-2
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-2
UniProt Gene Name
PRKAA2
UniProt Synonym Gene Names
AMPK; AMPK2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK2_HUMAN
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Product Notes
The AMPK prkaa2 (Catalog #AAA108352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AMPK can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AMPK prkaa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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