SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKC epsilon Recombinant Protein | PKCepsilon recombinant protein
PKC epsilon, Active
Applications
Western Blot
Synonyms
PKC epsilon; N/A; PKC epsilon, Active; PRKCE; MGC125656; MGC125657; nPKC-epsilon; PKCepsilon recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Applicable Applications for PKCepsilon recombinant protein
WB (Western Blot), Kinase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PKCepsilon recombinant protein
Recombinant full-length human PKC epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_005400.
PKCepsilon is a member of the protein kinase C (PKC) family of serine-and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKCepsilon is involved in many different cellular functions, such as neuron channel activation, cardioprotection from ischemia (1), heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may control anxiety-like behavior (2).
PKCepsilon is a member of the protein kinase C (PKC) family of serine-and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKCepsilon is involved in many different cellular functions, such as neuron channel activation, cardioprotection from ischemia (1), heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may control anxiety-like behavior (2).
Product Categories/Family for PKCepsilon recombinant protein
References
1. Chen, C H. et al: Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: role of epsilon protein kinase C. Proc. Nat. Acad. Sci. 96: 12784-12789, 1999.
2. Hodge, C W. et al: Decreased anxiety-like behavior, reduced stress hormones, and neurosteroid supersensitivity in mice lacking protein kinase C-epsilon. J. Clin. Invest. 110: 1003-1010, 2002.
2. Hodge, C W. et al: Decreased anxiety-like behavior, reduced stress hormones, and neurosteroid supersensitivity in mice lacking protein kinase C-epsilon. J. Clin. Invest. 110: 1003-1010, 2002.
NCBI and Uniprot Product Information
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Product Notes
The PKCepsilon (Catalog #AAA73863) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PKC epsilon can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the PKCepsilon for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC epsilon, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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