SDS-PAGE
Myosin Light Chain Kinase (MYLK) Recombinant Protein | MYLK recombinant protein
Recombinant Myosin Light Chain Kinase (MYLK)
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
> 95%
Synonyms
Myosin Light Chain Kinase (MYLK); N/A; Recombinant Myosin Light Chain Kinase (MYLK); MYLK recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose and preservative.
Sequence
FMGDNDNETL ANVTSATWDF DDEAFDEISD DAKDFISNLL KKDMKNRLDC TQCLQHPWLM KDTKNMEAKK LSKDRMKKYM ARRKWQKTGN AVRAIGRLSS MAMISGLSGR KSSTGSPTSP LNAEKLESEE DVSQAFLEAV AEEKPHVKPY FSKTIRDLEV VEGSAARFDC KIEGYPDPEV VWFKDDQSIR ESRHFQIDYD EDGNCSLIIS DVCGDDD
Sequence Length
1914
Applicable Applications for MYLK recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Phe1661~Asp1877
Subcellular Location
Cytoplasm. Cell projection, lamellipodium. Cleavage furrow. Cytoskeleton
Tags
Two N-terminal Tags, His-tag and GST-tag.
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
4.8
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26.2kDa
NCBI Official Full Name
myosin light chain kinase, smooth muscle isoform 1
NCBI Official Synonym Full Names
myosin light chain kinase
NCBI Official Symbol
MYLK
NCBI Official Synonym Symbols
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
NCBI Protein Information
myosin light chain kinase, smooth muscle; telokin; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
UniProt Protein Name
Myosin light chain kinase, smooth muscle
UniProt Gene Name
MYLK
UniProt Synonym Gene Names
MLCK; MLCK1; MYLK1; MLCK; smMLCK; KRP
UniProt Entry Name
MYLK_HUMAN
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Product Notes
The MYLK mylk (Catalog #AAA134218) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Myosin Light Chain Kinase (MYLK) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the MYLK mylk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FMGDNDNETL ANVTSATWDF DDEAFDEISD DAKDFISNLL KKDMKNRLDC TQCLQHPWLM KDTKNMEAKK LSKDRMKKYM ARRKWQKTGN AVRAIGRLSS MAMISGLSGR KSSTGSPTSP LNAEKLESEE DVSQAFLEAV AEEKPHVKPY FSKTIRDLEV VEGSAARFDC KIEGYPDPEV VWFKDDQSIR ESRHFQIDYD EDGNCSLIIS DVCGDDD. It is sometimes possible for the material contained within the vial of "Myosin Light Chain Kinase (MYLK), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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