Application Data
(AbFlex JMJD2A antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM JMJD2A antibody (rAb) antibody for JMJD2A. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM JMJD2A antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
Mouse anti-Human JMJD2A Antibody | anti-JMJD2A antibody
AbFlex JMJD2A antibody (rAb)
Gene Names
KDM4A; JMJD2; JHDM3A; JMJD2A; TDRD14A
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Protein A Chromatography
Synonyms
JMJD2A; N/A; AbFlex JMJD2A antibody (rAb); KDM4A; JMJD2; JHDM3A; TDRD14A; anti-JMJD2A antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 200mM Hepes pH7.5, 100mM NaCl, 50mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-JMJD2A antibody
WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Immunogen
This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(AbFlex JMJD2A antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM JMJD2A antibody (rAb) antibody for JMJD2A. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM JMJD2A antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
Application Data
(AbFlex JMJD2A antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM JMJD2A antibody (rAb) antibody for JMJD2A. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM JMJD2A antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
WB (Western Blot)
(AbFlex JMJD2A antibody (rAb) tested by Western blot. HeLa cell nuclear extract (20 ug) probed with AbFlex JMJD2A antibody (rAb) at a dilution of 2 ug/ml.)
WB (Western Blot)
(AbFlex JMJD2A antibody (rAb) tested by Western blot. HeLa cell nuclear extract (20 ug) probed with AbFlex JMJD2A antibody (rAb) at a dilution of 2 ug/ml.)
Related Product Information for anti-JMJD2A antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex JMJD2A antibody (rAb) was raised in a Mouse host. It has been validated for use in ELISA and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.
Product Categories/Family for anti-JMJD2A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,697 Da
NCBI Official Full Name
lysine-specific demethylase 4A
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4A
NCBI Official Symbol
KDM4A
NCBI Official Synonym Symbols
JMJD2; JHDM3A; JMJD2A; TDRD14A
NCBI Protein Information
lysine-specific demethylase 4A; jmjC domain-containing histone demethylation protein 3A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A; jumonji domain-containing protein 2A; tudor domain cont
UniProt Protein Name
Lysine-specific demethylase 4A
UniProt Gene Name
KDM4A
UniProt Synonym Gene Names
JHDM3A; JMJD2; JMJD2A; KIAA0677
UniProt Entry Name
KDM4A_HUMAN
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Product Notes
The JMJD2A kdm4a (Catalog #AAA60264) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex JMJD2A antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the JMJD2A kdm4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD2A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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