Mouse anti-Human CD57 Antibody | anti-CD57 antibody

Anti-Hu CD57 PE-Cy7

Cat. Num. AAA129346

• Gene Names: B3GAT1; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD57; N/A; Anti-Hu CD57 PE-Cy7; beta-1,3-glucuronyltransferase 1; HNK1, LEU7, 3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc; anti-CD57 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgM
• Clone Number: TB01
• Specificity: The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
• Purity/Purification: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

• Applicable Applications for anti-CD57 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: A pool of neuroblastoma cell lines
• Conjugation: PE-Cy7
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD3 positive CD57 positive T cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD57 (TB01) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD57 (TB01) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD57 (TB01) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD57 antibody

CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.

References

*Lim SA, Kim TJ, Lee JE, Sonn CH, Kim K, Kim J, Choi JG, Choi IK, Yun CO, Kim JH, Yee C, Kumar V, Lee KM: Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013 Apr 15;73(8):2598-607., URL: http://www.ncbi.nlm.nih.gov/pubmed/23580577,*Cunha KS, Caruso AC, Faria PA, Silva LE, Fonseca EC, Geller M, de Moura-Neto RS, Lopes VS: Evaluation of Bcl-2, Bcl-x and cleaved caspase-3 in malignant peripheral nerve sheath tumors and neurofibromas. An Acad Bras Cienc. 2013;85(4):1497-511., URL: http://www.ncbi.nlm.nih.gov/pubmed/24346802 ,*Phadwal K, Alegre-Abarrategui J, Watson AS, Pike L, Anbalagan S, Hammond EM, Wade-Martins R, McMichael A, Klenerman P, Simon AK: A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells. Autophagy. 2012 Apr;8(4):677-89., URL: http://www.ncbi.nlm.nih.gov/pubmed/22302009 ,*Marquardt N, Béziat V, Nystrom S, Hengst J, Ivarsson MA, Kekäläinen E, Johansson H, Mjosberg J, Westgren M, Lankisch TO, Wedemeyer H, Ellis EC, Ljunggren HG, Michaëlsson J, Bjorkstrom NK: Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015 Mar 15;194(6):2467-71., URL: https://pubmed.ncbi.nlm.nih.gov/25672754/,*Wangerin H, Kristiansen G, Schlomm T, Stephan C, Gunia S, Zimpfer A, Weichert W, Sauter G, Erbersdobler A: CD57 expression in incidental, clinically manifest, and metastatic carcinoma of the prostate. Biomed Res Int. 2014;2014:356427., URL: https://pubmed.ncbi.nlm.nih.gov/24977150/,*Fernandez S, French MA, Price P: Immunosenescent CD57+CD4+ T-cells accumulate and contribute to interferon-γ responses in HIV patients responding stably to ART. Dis Markers. 2011;31(6):337-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/22182806 ,

NCBI and Uniprot Product Information

• NCBI GI #: 77695914
• NCBI GeneID: 27087
• NCBI Accession #: NP_061114.2
• NCBI GenBank Nucleotide #: NP_061114.2
• UniProt Accession #: Q9P2W7
• NCBI Official Full Name: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: beta-1,3-glucuronyltransferase 1
• NCBI Official Symbol: B3GAT1
• NCBI Official Synonym Symbols: NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• NCBI Protein Information: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• UniProt Protein Name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• UniProt Gene Name: B3GAT1
• UniProt Synonym Gene Names: GlcUAT-P