Standard Curve (Sample)
Mouse Complement component C8 alpha chain (C8A) ELISA Kit | C8A elisa kit
Mouse Complement component C8 alpha chain (C8A) ELISA Kit
Reactivity
Mouse
Synonyms
Complement component C8 alpha chain (C8A); N/A; Mouse Complement component C8 alpha chain (C8A) ELISA Kit; C8A elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for C8A elisa kit
Intended Uses: This C8alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C8alpha. This ELISA kit for research use only!
Principle of the Assay||C8alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C8alpha antibody and an C8alpha-HRP conjugate. The assay sample and buffer are incubated together with C8alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C8alpha concentration since C8alpha from samples and C8alpha-HRP conjugate compete for the anti-C8alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by C8alpha from the sample, fewer sites are left to bind C8alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C8alpha concentration in each sample is interpolated from this standard curve.
Principle of the Assay||C8alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C8alpha antibody and an C8alpha-HRP conjugate. The assay sample and buffer are incubated together with C8alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C8alpha concentration since C8alpha from samples and C8alpha-HRP conjugate compete for the anti-C8alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by C8alpha from the sample, fewer sites are left to bind C8alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C8alpha concentration in each sample is interpolated from this standard curve.
Product Categories/Family for C8A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,163 Da
NCBI Official Full Name
complement component C8 alpha chain
NCBI Official Synonym Full Names
complement component 8, alpha polypeptide
NCBI Official Symbol
C8A
NCBI Protein Information
complement component C8 alpha chain; complement component 8 subunit alpha
UniProt Protein Name
Complement component C8 alpha chain
UniProt Gene Name
C8A
UniProt Entry Name
CO8A_HUMAN
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Product Notes
The Mouse C8A c8a (Catalog #AAA245781) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245781 ELISA Kit recognizes Mouse C8A. It is sometimes possible for the material contained within the vial of "Complement component C8 alpha chain (C8A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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