IF (Immunofluorescence)
(Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody 2D10. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of 2D10 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
VAP-1 recombinant antibody
Anti-VAP-1 [2D10]
Gene Names
AOC3; HPAO; SSAO; VAP1; VAP-1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Protein A Affinity Purified
Synonyms
VAP-1, Recombinant Antibody; Anti-VAP-1 [2D10]; Vascular adhesion protein 1; VAP1; Copper amine oxidase; HPAO; Membrane primary amine oxidase; Semicarbazide-sensitive; AOC3; Amine oxidase, copper containing 3; amine oxidase; SSAO; copper-containing amine:oxygen oxidoreductase; VAP-1 recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
2D10
Specificity
Recognizes human Vascular Adhesion Protein-1 (VAP-1), a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. 2D10 is a conformational specific antibody which recognizes an epitope present only in the dimeric form of VAP-1. Elevated soluble VAP-1 serum- levels have been described in several inflammatory- diseases as well as colorectal cancer.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
763
Applicable Applications for VAP-1 recombinant antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
Immunogen
Affinity purified VAP-1 from tonsil stroma of human origin
Note
Describes the production of monoclonal antibody against VAP-1 (2D10) and its use in immunoblotting to identify that circulating soluble VAP-1 in serum exists primarily as a dimer.
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
IF (Immunofluorescence)
(Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody 2D10. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of 2D10 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody 2D10. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of 2D10 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Product Categories/Family for VAP-1 recombinant antibody
References
Kurkijärvi R, Adams DH, Leino R, Möttönen T, Jalkanen S, Salmi M. Circulating form of human vascular adhesion protein-1 (VAP-1): increased serum levels in inflammatory liver diseases. J Immunol. 1998 Aug 1;161(3):1549-57. PMID:9686623
Original Published Journal: J Immunol. 1998 Aug 1;161(3):1549-57.
Publication Note: Describes the production of monoclonal antibody against VAP-1 (2D10) and its use in immunoblotting to identify that circulating soluble VAP-1 in serum exists primarily as a dimer.
Original Published Journal: J Immunol. 1998 Aug 1;161(3):1549-57.
Publication Note: Describes the production of monoclonal antibody against VAP-1 (2D10) and its use in immunoblotting to identify that circulating soluble VAP-1 in serum exists primarily as a dimer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Membrane primary amine oxidase
NCBI Official Synonym Full Names
amine oxidase copper containing 3
NCBI Official Symbol
AOC3
NCBI Official Synonym Symbols
HPAO; SSAO; VAP1; VAP-1
NCBI Protein Information
membrane primary amine oxidase
UniProt Protein Name
Membrane primary amine oxidase
UniProt Gene Name
AOC3
UniProt Synonym Gene Names
VAP1; SSAO; VAP-1
UniProt Entry Name
AOC3_HUMAN
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Product Notes
The VAP-1 aoc3 (Catalog #AAA72018) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-VAP-1 [2D10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VAP-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the VAP-1 aoc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAP-1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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