TFEB recombinant antibody

Rabbit anti-TFEB Recombinant Monoclonal Antibody [BLR070G]

Cat. Num. AAA213560

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: TFEB, Recombinant Antibody; Rabbit anti-TFEB Recombinant Monoclonal Antibody [BLR070G]; ALPHATFEB; transcription factor EB; TCFEB; BHLHE35; class E basic helix-loop-helix protein 35; T-cell transcription factor EB; Transcription factor EB; TFEB recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR070G
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for TFEB recombinant antibody: FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 426 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human TFEB by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, MCF-7, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of mouse TFEB by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560 lot 2) used for IP at 20 ul/mg lysate. TFEB was also immunoprecipitated by a previous lot of this antibody (AAA213560 lot 1) and a second antibody against a different epitope of TFEB . For blotting immunoprecipitated TFEB, AAA213560 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

FCM/FACS (Flow Cytometry)

(Detection of human TFEB (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-TFEB recombinant monoclonal antibody (AAA213560) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for TFEB recombinant antibody

Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression; thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19484].

Product Categories/Family for TFEB recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 268607698
• NCBI GeneID: 7942
• NCBI Accession #: NP_009093.1
• NCBI GenBank Nucleotide #: NM_007162
• UniProt Accession #: P19484; Q709B3; Q7Z6P9; Q9BRJ5; Q9UJD8
• NCBI Official Full Name: Homo sapiens transcription factor EB (TFEB), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• UniProt Protein Name: Transcription factor EB
• UniProt Gene Name: TFEB
• UniProt Synonym Gene Names: BHLHE35; bHLHe35
• UniProt Entry Name: TFEB_HUMAN