IF (Immunofluorescence)
(ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human ST13 Monoclonal Antibody | anti-ST13 antibody
ST13 antibody
Gene Names
ST13; HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
ST13, Antibody; ST13 antibody; Hsc70-interacting protein; ST13; HOP; SNC6; FAM10A1; anti-ST13 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT5C6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
369
Applicable Applications for anti-ST13 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human HIP/ST13 (1-369aa) purified from E. coli
Clone Note
Anti-human ST13 mAb, clone AT5C6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ST13 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ST13 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HepG2 cell lysate Lane 3.: SW480 cell lysate Lane 4.: Jurkat cell lysate Lane 5.: K562 cell lysate Lane 6.: LnCap cell lysate Lane 7.: HeLa cell lysate Lane 8.: PC3 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ST13 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HepG2 cell lysate Lane 3.: SW480 cell lysate Lane 4.: Jurkat cell lysate Lane 5.: K562 cell lysate Lane 6.: LnCap cell lysate Lane 7.: HeLa cell lysate Lane 8.: PC3 cell lysate)
Related Product Information for anti-ST13 antibody
HIP (Hsc70-interacting protein), also known as ST13, is a co-chaperone to the major heat shock proteins, HSP70 and HSP90, and appears in early receptor complexes. Through mutual binding to both HSP70 and HSP90, Hip functions as an adaptor that can integrate HSP70 and HSP90 interactions. Also, Hip has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones.
Product Categories/Family for anti-ST13 antibody
References
Nelson GM, et al., (2004) Mol Endocrinol.18 (7): 1620-30; Johnson BD, et al., (1998) J Biol Chem. 273 (6):3679-86
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,332 Da
NCBI Official Full Name
hsc70-interacting protein isoform 1
NCBI Official Synonym Full Names
ST13, Hsp70 interacting protein
NCBI Official Symbol
ST13
NCBI Official Synonym Symbols
HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
NCBI Protein Information
hsc70-interacting protein
UniProt Protein Name
Hsc70-interacting protein
UniProt Gene Name
ST13
UniProt Synonym Gene Names
AAG2; FAM10A1; HIP; SNC6; Hip
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Product Notes
The ST13 st13 (Catalog #AAA48146) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ST13 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ST13 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ST13 st13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ST13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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