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product-image-AAA249053_WB11.jpg WB (Western Blot) (HeLa cells were subjected to SDS PAGE followed by western blot with AAA249053 (PARP1 Antibody) at dilution of 1:20000)

Mouse PARP1 Monoclonal Antibody | anti-PARP1 antibody

Anti-PARP1 Antibody

Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>=95% as determined by SDS-PAGE
Protein A+G Purified
Synonyms
PARP1, Antibody; Anti-PARP1 Antibody; ADPRT; ADPRT 1; ADPRT1; pADPRT 1; PARP; PARP 1; PARP1; poly (ADP ribose) polymerase 1; Poly [ADP ribose] polymerase 1; Poly[ADP ribose] synthase 1; PPOL; anti-PARP1 antibody
Ordering
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
>=95% as determined by SDS-PAGE
Protein A+G Purified
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Sequence Length
1014
Applicable Applications for anti-PARP1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Poly (ADP-ribose) polymerase 1
Preparation and Storage
Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

WB (Western Blot)

(HeLa cells were subjected to SDS PAGE followed by western blot with AAA249053 (PARP1 Antibody) at dilution of 1:20000)

product-image-AAA249053_WB11.jpg WB (Western Blot) (HeLa cells were subjected to SDS PAGE followed by western blot with AAA249053 (PARP1 Antibody) at dilution of 1:20000)

IF (Immunofluorescence)

(Immunofluorescent analysis of HeLa cells using AAA249053 (PARP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L))

product-image-AAA249053_IF13.jpg IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells using AAA249053 (PARP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human breast cancer using AAA249053 (PARP1 antibody) at dilution of 1:600)

product-image-AAA249053_IHC15.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using AAA249053 (PARP1 antibody) at dilution of 1:600)
Related Product Information for anti-PARP1 antibody
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
142
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
115 kDa; 89 kDa
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly(ADP-ribose) polymerase 1
NCBI Official Symbol
PARP1
NCBI Official Synonym Symbols
PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
NCBI Protein Information
poly [ADP-ribose] polymerase 1
UniProt Protein Name
Poly [ADP-ribose] polymerase 1
UniProt Gene Name
PARP1
UniProt Synonym Gene Names
ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
UniProt Entry Name
PARP1_HUMAN

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Product Notes

The PARP1 parp1 (Catalog #AAA249053) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PARP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PARP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PARP1 parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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