IF (Immunofluorescence)
(ICC/IF analysis of LRPAP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LRPAP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human LRPAP1 Monoclonal Antibody | anti-LRPAP1 antibody
LRPAP1 antibody
Gene Names
LRPAP1; RAP; MRAP; A2RAP; HBP44; MYP23; A2MRAP; alpha-2-MRAP
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
LRPAP1, Antibody; LRPAP1 antibody; alpha-2-macroglobulin receptor-associated protein; A2MRAP; A2RAP; HBP44; MGC138272; MRAP; RAP; anti-LRPAP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT8G8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
357
Applicable Applications for anti-LRPAP1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human LRPAP1 (35-357aa) purified from E. coli
Cross reactivity
mouse
Clone Note
Anti-human LRPAP1 mAb, clone AT8G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LRPAP1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of LRPAP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LRPAP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of LRPAP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LRPAP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The lysate of Mouse kidney (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The lysate of Mouse kidney (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-LRPAP1 antibody
Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. LRPAP1 is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains.LRPAP1 is present on the cell surface, where it is an effective inhibitor of ligand binding to the kidney glycoprotein 330 (gp330) yet it is most abundant in the endoplasmic reticulum lumen and may function in receptor folding and/or trafficking.
Product Categories/Family for anti-LRPAP1 antibody
References
Kounnas. M.Z., et al. (1992) J Biol Chem 267 (29): 21162-21166; Bu. G., et al. (1995) EMBO J 14 (10): 2269-2280
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,466 Da
NCBI Official Full Name
alpha-2-macroglobulin receptor-associated protein
NCBI Official Synonym Full Names
LDL receptor related protein associated protein 1
NCBI Official Symbol
LRPAP1
NCBI Official Synonym Symbols
RAP; MRAP; A2RAP; HBP44; MYP23; A2MRAP; alpha-2-MRAP
NCBI Protein Information
alpha-2-macroglobulin receptor-associated protein
UniProt Protein Name
Alpha-2-macroglobulin receptor-associated protein
UniProt Gene Name
LRPAP1
UniProt Synonym Gene Names
A2MRAP; Alpha-2-MRAP; RAP
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Product Notes
The LRPAP1 lrpap1 (Catalog #AAA48150) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LRPAP1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LRPAP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the LRPAP1 lrpap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRPAP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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