Mouse anti-Human KI67 Monoclonal Antibody | anti-KI67 antibody

KI67 Antibody

Cat. Num. AAA322665

• Gene Names: MKI67; KIA; MIB-; MIB-1; PPP1R105
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity-Chromatography
• Synonyms: KI67, Antibody; KI67 Antibody; Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen KI67; Antigen Ki67; KI67_HUMAN; KIA; Marker of proliferation Ki-67; MIB 1; MIB; MKI67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2; anti-KI67 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 2896

• Applicable Applications for anti-KI67 antibody: ELISA, IHC (Immunohistochemistry)
• Immunogen: Purified recombinant fragment of human KI67 expressed in E. Coli
• Preparation and Storage: Store at -20 degrees C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(Figure 1: Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D), showing nuclear localization using KI67 mouse mAb with DAB staining.)

Related Product Information for anti-KI67 antibody

Description: Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Function: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Subunit Structure: Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity (PubMed:19131338, PubMed:23178126).
Post-translational Modifications: Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

NCBI and Uniprot Product Information

• NCBI GI #: 225543215
• NCBI GeneID: 4288
• NCBI Accession #: NP_001139438.1
• NCBI GenBank Nucleotide #: NM_001145966.1
• UniProt Accession #: P46013; Q5VWH2
• Molecular Weight: Observed: 358 kDa; Predicted: 359 kDa
• NCBI Official Full Name: proliferation marker protein Ki-67 isoform 2
• NCBI Official Synonym Full Names: marker of proliferation Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA; MIB-; MIB-1; PPP1R105
• NCBI Protein Information: proliferation marker protein Ki-67
• UniProt Protein Name: Proliferation marker protein Ki-67
• UniProt Gene Name: MKI67
• UniProt Synonym Gene Names: Antigen KI-67Curated; Antigen Ki67Curated