Mouse anti-Human KAP1 / TIF1 beta Monoclonal Antibody | anti-TRIM28 antibody

KAP1 / TIF1 beta Antibody

Cat. Num. AAA290520

• Gene Names: TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, Western Blot
• Synonyms: KAP1 / TIF1 beta, Antibody; KAP1 / TIF1 beta Antibody; Purified Mouse Monoclonal Antibody (Mab); E3 SUMO protein ligase TRIM28; E3 SUMO-protein ligase TRIM28; FLJ29029; KAP 1; KAP-1; KRAB associated protein 1; KRAB interacting protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP 1; KRIP-1; KRIP1; Nuclear corepressor KAP 1; Nuclear corepressor KAP-1; RING finger protein 96; RNF96; TF1B; TIF1 beta; TIF1-beta; TIF1B; TIF1B_HUMAN; Transcription intermediary factor 1 beta; Transcription intermediary factor 1-beta; TRIM28; Tripartite motif containing 28; tripartite motif containing protein 28; Tripartite motif-containing protein 28.; anti-TRIM28 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Sequence Length: 835

• Applicable Applications for anti-TRIM28 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(IHC of paraffin-embedded human Spleen using anti-KAP1 / TIF1 beta diluted 1/500-1/1000.)

IP (Immunoprecipitation)

(Immunoprecipitation analysis of Hela cell lysates using KAP1 / TIF1 beta mouse mAb.)

ICC (Immunocytochemistry)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KAP1 / TIF1 beta mouse mAb (dilution 1:100).)

WB (Western Blot)

(Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.)

Related Product Information for anti-TRIM28 antibody

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors.

Product Categories/Family for anti-TRIM28 antibody

Cancer; Cell biology

References

Friedman J.R.,et al.Genes Dev. 10:2067-2078(1996). Moosmann P.R.,et al.Nucleic Acids Res. 24:4859-4867(1996). Emison E.S.,et al.Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases. Bienvenut W.V.,et al.Submitted (MAY-2006) to UniProtKB. Bienvenut W.V.,et al.Submitted (JAN-2010) to UniProtKB.

NCBI and Uniprot Product Information

• NCBI GI #: 5032179
• NCBI GeneID: 10155
• NCBI Accession #: NP_005753.1
• NCBI GenBank Nucleotide #: NM_005762.2
• UniProt Accession #: Q13263; O00677; Q7Z632; Q93040; Q96IM1
• Molecular Weight: 88550
• NCBI Official Full Name: transcription intermediary factor 1-beta
• NCBI Official Synonym Full Names: tripartite motif containing 28
• NCBI Official Symbol: TRIM28
• NCBI Official Synonym Symbols: KAP1; TF1B; RNF96; TIF1B; PPP1R157
• NCBI Protein Information: transcription intermediary factor 1-beta
• UniProt Protein Name: Transcription intermediary factor 1-beta
• UniProt Gene Name: TRIM28
• UniProt Synonym Gene Names: KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
• UniProt Entry Name: TIF1B_HUMAN