FCM/FACS (Flow Cytometry)
(The graph on the right shows B-K8 with non-activated monocytes. In the two cases, the dotted line represents the IgG1 isotype control.)
Mouse anti-Human IL-8 Monoclonal Antibody | anti-IL-8 antibody
Mouse Anti-Human IL-8 Monoclonal Antibody,Azide Free Clone B-K8
Gene Names
IL8; CXCL8; AMCF-I
Reactivity
Human
Applications
Functional Assay, ELISA, Flow Cytometry, Functional Assay
Purity
Ion exchange chromatography
Synonyms
IL-8, Antibody; Mouse Anti-Human IL-8 Monoclonal Antibody,Azide Free Clone B-K8; IL-8; anti-IL-8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 Kappa light chain
Clone Number
B-K8
Specificity
Recognises both natural and recombinant human IL-8
Purity/Purification
Ion exchange chromatography
Form/Format
Phosphate-buffered saline. Sterile-filtered through 0.22 um. Carrier and preservative free
Applicable Applications for anti-IL-8 antibody
FA (Functional Assay), ELISA, FCM/FACS (Flow Cytometry)
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunization
Recombinant human IL-8
Biological Activity
Inhibits IL-8 induced chemotaxis of human neutrophils.
Preparation and Storage
Store at +2-8 degree C for 12 months. For longer storage, freeze aliquots.
FCM/FACS (Flow Cytometry)
(The graph on the right shows B-K8 with non-activated monocytes. In the two cases, the dotted line represents the IgG1 isotype control.)
FCM/FACS (Flow Cytometry)
(The graph on the right shows B-K8 with non-activated monocytes. In the two cases, the dotted line represents the IgG1 isotype control.)
FCM/FACS (Flow Cytometry)
(The graph on the left represents activated monocytes marked with B-K8.)
FCM/FACS (Flow Cytometry)
(The graph on the left represents activated monocytes marked with B-K8.)
Related Product Information for anti-IL-8 antibody
Interleukin 8 (IL-8) or CXCL8, Monocyte-Derived Neutrophil Chemotactic Factor (MDNCF), Neutrophil Activating Factor (NAF) and NAD-P1 is a chemokine secreted by monocytes, macrophages and endothelial cells. IL-8 chemoattracts and activates neutrophils.
The predominant form of IL-8 is a 8.4kDa protein containing 72 amino acid residues, which includes five additional N-Terminal amino-acids. IL-8 contains the four conserved cysteine residues present in CXC chemokines and also contains the “ELR” motif common to CXC chemokines that binds to CXCR1 and CXCR2.
Data indicate that IL-8 may participate in the pathogenesis of rheumatoid arthritis via the induction of neutrophil-mediated cartilage damage, and psoriasis. A causative involvement of IL-8 is found within a broad range of clinico-pathological conditions : adult respiratory distress syndrome, asthma, bacterial infections, bladder cancer, graft rejection and influenza infection, due to the now known biological properties of IL-8. This cytokine especially in combinations with other neutrophil activating agents, may prove helpful in the treatment of patients suffering from granulocytopenia, severe infections against which antibiotics are not effective, and immunodeficiency caused by HIV.
The predominant form of IL-8 is a 8.4kDa protein containing 72 amino acid residues, which includes five additional N-Terminal amino-acids. IL-8 contains the four conserved cysteine residues present in CXC chemokines and also contains the “ELR” motif common to CXC chemokines that binds to CXCR1 and CXCR2.
Data indicate that IL-8 may participate in the pathogenesis of rheumatoid arthritis via the induction of neutrophil-mediated cartilage damage, and psoriasis. A causative involvement of IL-8 is found within a broad range of clinico-pathological conditions : adult respiratory distress syndrome, asthma, bacterial infections, bladder cancer, graft rejection and influenza infection, due to the now known biological properties of IL-8. This cytokine especially in combinations with other neutrophil activating agents, may prove helpful in the treatment of patients suffering from granulocytopenia, severe infections against which antibiotics are not effective, and immunodeficiency caused by HIV.
References
Schruefer et.al Am J Physiol Heart Ciec Physiol 2005
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-8
NCBI Official Symbol
IL8
NCBI Official Synonym Symbols
CXCL8; AMCF-I
NCBI Protein Information
interleukin-8
UniProt Protein Name
Interleukin-8
UniProt Gene Name
CXCL8
UniProt Synonym Gene Names
IL8; IL-8; AMCF-I
UniProt Entry Name
IL8_PIG
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Product Notes
The IL-8 cxcl8 (Catalog #AAA58559) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IL-8 Monoclonal Antibody,Azide Free Clone B-K8 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-8 can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IL-8 cxcl8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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