Rat Heparan Sulphate Proteoglycan Monoclonal Antibody | anti-GPC3 antibody

Rat anti Heparan Sulphate Proteoglycan

Cat. Num. AAA77481

• Gene Names: GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; GPC3
• Reactivity: Bovine, Human, Mouse, Rat, Cod and Wolffish.
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Synonyms: Heparan Sulphate Proteoglycan, Antibody; Rat anti Heparan Sulphate Proteoglycan; anti-GPC3 antibody

• Host: Rat
• Reactivity: Bovine, Human, Mouse, Rat, Cod and Wolffish.
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: A7L6
• Specificity: A7L6 recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.
• Form/Format: The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-GPC3 antibody: WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
• Source Note: A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
• Warning and Caution: This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Application Data

(Figure 1: Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.)

Related Product Information for anti-GPC3 antibody

Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keRatan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is loCated predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.

Product Categories/Family for anti-GPC3 antibody

Extracellular matrix

References

Couchman, J.R. (1987). Heterogeneous distribution of a basement membrane heparin sulfate proteoglycan in Rat tissues. The Journal of Cell Biology 105, 1901-16. Couchman, J. R., and Ljubimov, A. V. (1989). Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix 9, 311-21. 3. Horiguchi, Y., Couchman, J. R., Ljubimov, A. V., Yamasaki, H., and Fine, J. D. (1989). Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in Human skin and other basement membranes. J Histochem Cytochem 37, 961-70. Ljubimov, A. V., Bartek, J., Couchman, J. R., Kapuller, L. L., Veselov, V. V., Kovarik, J., Perevoshchikov, A. G., and Krutovskikh, V. A. (1992). Distribution of individual components of basement membrane in Human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50, 562-66. Couchman, J. R., Ljubimov, A. V., Sthanam, M., Horchar, T., and Hassell, J. R. (1995). Antibody mapping and tissue localization of globular and cysteine-rich regions of perlecan domain III. J Histochem Cytochem 43, 955-63. Tapanadechopone, P., Hassell, J. R., Rigatti, B., and Couchman, J. R. (1999). Localization of glycosaminoglycan substitution sites on domain V of Mouse perlecan. Biochem Biophys Res Commun 265, 680-90. Tingbø, M. G., Kolset, S. O., Ofstad, R., Enersen, G., Hannesson, K. O. (2006). IdentifiCation and distribution of heparan sulfate proteoglycans in the white muscle of Atlantic cod (Gadus morhua) and spotted wolffish (Anarhichas minor). CompaRative Biochemistry and Physiology Part B 143,441-52.

NCBI and Uniprot Product Information

• NCBI GI #: 1245417
• NCBI GeneID: 2719
• UniProt Accession #: P51654; Q2L880; Q2L882; Q53H15; Q8IYG2
• NCBI Official Full Name: heparan sulphate proteoglycan
• NCBI Official Synonym Full Names: glypican 3
• NCBI Official Symbol: GPC3
• NCBI Official Synonym Symbols: SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; GPC3
• NCBI Protein Information: glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan
• UniProt Protein Name: Glypican-3
• UniProt Gene Name: GPC3
• UniProt Synonym Gene Names: OCI5
• UniProt Entry Name: GPC3_HUMAN