IHC (Immunohiostchemistry)
(AAA290837 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Mouse anti-Human, Mouse GLS2 Monoclonal Antibody | anti-GLS2 antibody
GLS2 Antibody
Gene Names
GLS2; GA; GLS; LGA; hLGA
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
GLS2, Antibody; GLS2 Antibody; Glutaminase liver isoform; mitochondrial; GLS; 3.5.1.2; L-glutaminase; L-glutamine amidohydrolase; GLS2; GA; anti-GLS2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This GLS2 antibody is generated from a mouse immunized with a recombinant protein of human GLS2.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
337
Applicable Applications for anti-GLS2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Mitochondrion
Tissue Location
Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
Function
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha- ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(AAA290837 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA290837 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
WB (Western Blot)
(All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilutionLane 1: human brain lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilutionLane 1: human brain lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GLS2 antibody
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Product Categories/Family for anti-GLS2 antibody
References
Gomez-Fabre P.M.,et al.Biochem. J. 345:365-375 (2000). Perez-Gomez C.,et al.Biochem. J. 370:771-784 (2003). Chavez R.A.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45 (2004). Scherer S.E.,et al.Nature 440:346-351 (2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66323 Da
NCBI Official Full Name
glutaminase liver isoform, mitochondrial isoform 4
NCBI Official Synonym Full Names
glutaminase 2
NCBI Official Symbol
GLS2
NCBI Official Synonym Symbols
GA; GLS; LGA; hLGA
NCBI Protein Information
glutaminase liver isoform, mitochondrial
UniProt Protein Name
Glutaminase liver isoform, mitochondrial
UniProt Gene Name
GLS2
UniProt Synonym Gene Names
GA; GLS
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Product Notes
The GLS2 gls2 (Catalog #AAA290837) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GLS2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLS2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GLS2 gls2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLS2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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