Mouse anti-Human, rat Fibrillarin Monoclonal Antibody | anti-NOP1 antibody

Anti-Fibrillarin (Nop1p)

Cat. Num. AAA72745

• Gene Names: NOP1; LOT3
• Reactivity: Human, rat
• Applications: Immunofluorescence, Western Blot
• Purity: Total IgG fraction
• Synonyms: Fibrillarin, Antibody; Anti-Fibrillarin (Nop1p); anti-NOP1 antibody

• Host: Mouse
• Reactivity: Human, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 38F3
• Specificity: Specific for the ~34k Fibrillarin protein in Western Blots of human, rat, plant, Drosophila, C. elegans and S. pombe. It is expected that it will work on other mammal tissues.
• Purity/Purification: Total IgG fraction
• Form/Format: Liquid. Contains 10 mM sodium azide.
• Sequence Length: 327

• Applicable Applications for anti-NOP1 antibody: IF (Immunofluorescence), WB (Western Blot)
• Antigen: Yeast nuclear preparations.
• Immunogen Information: Yeast nuclear preparations
• Immunogen Species: Yeast
• Packing: 100ul antibody per vial+10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.
• Species Reactivity Note: The antibody has been directly tested for reactivity in human, rat, plant, Drosophila, C. elegans and S. pombe. It is expected that it will work on other mammal tissues.
• Biological Significance: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
• Preparation and Storage: Store at -20 degree C: stable for at least 1 year.

Application Data

Related Product Information for anti-NOP1 antibody

Mouse monoclonal antibody

References

• Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123-133 (1985).
• Aris JP and Blobel G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107:17-31 (1988).
• Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 23:8519-8527 (2003).
• Tyagi S and Alsmadi O. Imaging native beta-actin mRNA in motile fibroblasts. Biophys J. 87:4153-62 (2004).
• Paeschke1 K, Simonsson T, Postberg J, Rhodes D, Lipps H-J. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo Nature Structural & Molecular Biology 12, 847-854 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 6320190
• NCBI GeneID: 851548
• NCBI Accession #: NP_010270.1
• NCBI GenBank Nucleotide #: NM_001180073.1
• UniProt Accession #: P15646; P89890; D6VRX5
• NCBI Official Full Name: Nop1p
• NCBI Official Symbol: NOP1
• NCBI Official Synonym Symbols: LOT3
• NCBI Protein Information: Nop1p
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• UniProt Gene Name: NOP1
• UniProt Synonym Gene Names: LOT3; Nucleolar protein 1
• UniProt Entry Name: FBRL_YEAST