IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).)
Mouse EMI1 (Early Mitotic Inhibitor-1) Monoclonal Antibody | anti-FBXO5 antibody
EMI1 (Early Mitotic Inhibitor-1) Mouse Monoclonal Antibody
Gene Names
FBXO5; EMI1; FBX5; Fbxo31
Reactivity
Human.Others not known
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
EMI1 (Early Mitotic Inhibitor-1), Antibody; EMI1 (Early Mitotic Inhibitor-1) Mouse Monoclonal Antibody; Early Mitotic Inhibitor-1 (EMI1); FBX5; Fbxo31; anti-FBXO5 antibody
Host
Mouse
Reactivity
Human.
Others not known
Others not known
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
EMI1/1176
Specificity
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
401
Applicable Applications for anti-FBXO5 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Nuclear
Immunogen
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Hu-Chromosome Location
6q25.2
Positive Control
HeLa, 293 or HepG2 cells. Ovarian carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).)
References
Reimann, J.D., et al. 2001. Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Cell 105: 645-655.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56kDa
NCBI Official Full Name
F-box only protein 5 isoform b
NCBI Official Synonym Full Names
F-box protein 5
NCBI Official Symbol
FBXO5
NCBI Official Synonym Symbols
EMI1; FBX5; Fbxo31
NCBI Protein Information
F-box only protein 5
UniProt Protein Name
F-box only protein 5
UniProt Gene Name
FBXO5
UniProt Synonym Gene Names
EMI1; FBX5
UniProt Entry Name
FBX5_HUMAN
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Product Notes
The FBXO5 fbxo5 (Catalog #AAA62675) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EMI1 (Early Mitotic Inhibitor-1) Mouse Monoclonal Antibody reacts with Human. Others not known and may cross-react with other species as described in the data sheet. AAA Biotech's EMI1 (Early Mitotic Inhibitor-1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the FBXO5 fbxo5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EMI1 (Early Mitotic Inhibitor-1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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