IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.)
Mouse anti-Human E2F-1 Monoclonal Antibody | anti-E2F1 antibody
E2F-1 Antibody
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Western Blot
Synonyms
E2F-1, Antibody; E2F-1 Antibody; Purified Mouse Monoclonal Antibody (Mab); Dmel\CG6376; Dmel_CG6376; drosE2F1; E(Sev-CycE)3A; E(var)3-93E; E2-promoter binding facto; E2F 1; E2F transcription factor 1; E2F-1; E2f-PA; E2f-PB; E2f-PC; E2F1; E2f1 E2F transcription factor 1; E2F1_HUMAN; Evar(3)164; KIAA4009; l(3)07172; l(3)j3B1; l(3)j3C2; l(3)rM729; mKIAA4009; OTTHUMP00000030661; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1.; anti-E2F1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Form/Format
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Sequence Length
437
Applicable Applications for anti-E2F1 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
Antigen Source
Human
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.)
ICC (Immunocytochemistry)
(Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).)
ICC (Immunocytochemistry)
(Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).)
WB (Western Blot)
(Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
WB (Western Blot)
(Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Related Product Information for anti-E2F1 antibody
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
Product Categories/Family for anti-E2F1 antibody
References
Helin K.,et al.Cell 70:337-350(1992). Kaelin W.G. Jr.,et al.Cell 70:351-364(1992). Shan B.,et al.Mol. Cell. Biol. 12:5620-5631(1992). Neuman E.,et al.Gene 173:163-169(1996). Deloukas P.,et al.Nature 414:865-871(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46920
NCBI Official Full Name
transcription factor E2F1
NCBI Official Synonym Full Names
E2F transcription factor 1
NCBI Official Symbol
E2F1
NCBI Official Synonym Symbols
RBP3; E2F-1; RBAP1; RBBP3
NCBI Protein Information
transcription factor E2F1
UniProt Protein Name
Transcription factor E2F1
UniProt Gene Name
E2F1
UniProt Synonym Gene Names
RBBP3; E2F-1; RBAP-1; RBBP-3
UniProt Entry Name
E2F1_HUMAN
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Product Notes
The E2F1 e2f1 (Catalog #AAA290480) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The E2F-1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's E2F-1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the E2F1 e2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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