Mouse Cytokeratin 7/17 Monoclonal Antibody | anti-KRT7/KRT17 antibody

Cytokeratin 7/17

Cat. Num. AAA215330

• Gene Names: KRT7; K7; CK7; SCL; K2C7
• Reactivity: Human, Cow; Does not react with Mouse, Rat, Ferret or Rabbit.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cytokeratin 7/17, Antibody; Cytokeratin 7/17; CK-7; K2C7; Keratin 55K Type II Cytoskeletal; Keratin Simple Epithelial Type 1 K7; Keratin Type II Cytoskeletal 7; Krt2-7; KRT7; Sarcolectin; SCL; Type II Mesothelial Keratin K7; Type-II Keratin Kb7; CK17; K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1; anti-KRT7/KRT17 antibody

• Host: Mouse
• Reactivity: Human, Cow; Does not react with Mouse, Rat, Ferret or Rabbit.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: C-46
• Specificity: It recognizes intermediate filament proteins (IFP's) of 55kDa and 46kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 469

• Applicable Applications for anti-KRT7/KRT17 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Positive Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.
• Immunogen: Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 12q13.13 (CK7) & 17q21.2 (CK17)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Cytokeratin 7/17 Mouse Monoclonal Antibody (C-46). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7/17 Mouse Monoclonal Antibody (C-46).)

References

1. Bartkova et al. Neoplasma 38(4), 439-46 (1991). 2. Vojtesek B, Staskova Z, Nenutil R, Bartkova J, Kovarik J, Rejthar A, Bartek J: A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma. 1990;37(3):333-42. 3. Bartek J et. al. Journal of Pathology, 1991, 164(3): 215-24

NCBI and Uniprot Product Information

• NCBI GI #: 67782365
• NCBI GeneID: 3855
• NCBI Accession #: NP_005547.3
• NCBI GenBank Nucleotide #: NP_005547.3
• UniProt Accession #: P08729; Q04695
• Molecular Weight: 55kDa (CK7); 46kDa (CK17)
• NCBI Official Full Name: keratin, type II cytoskeletal 7
• NCBI Official Synonym Full Names: keratin 7
• NCBI Official Symbol: KRT7
• NCBI Official Synonym Symbols: K7; CK7; SCL; K2C7
• NCBI Protein Information: keratin, type II cytoskeletal 7
• UniProt Protein Name: Keratin, type II cytoskeletal 7
• UniProt Gene Name: KRT7
• UniProt Synonym Gene Names: SCL; CK-7; K7
• UniProt Entry Name: K2C7_HUMAN